LOCUS       sequence-163957-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control for beta-catenin reporter. Contains mutated TCF/LEF binding 
            sites upstream of a luciferase reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Veeman MT, Slusarski DC, Kaykas A, Louie SH, Moon RT
  TITLE     Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, 
            regulates gastrulation movements.
  JOURNAL   Curr Biol. 2003 Apr 15. 13(8):680-5.
  PUBMED    12699626
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Apr 15. 13(8):680-5."
COMMENT     Sequence Label: M51 Super 8x FOPFlash (TOPFlash mutant)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(91..122)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    279..370
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(311..330)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    384..432
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(700..721)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc ggaatgccaa
       61 gctttttacc aacagtaccg gaatgccaag ctggaattcg agcttccatt atataccctc
      121 tagagtctag atctcgagcc cgggcctttg gccttacccc ctttggcctt accccctttg
      181 gccttacccc ctttggcctc gcgcctttgg ccttaccccc tttggcctta ccccctttgg
      241 ccttaccccc tttggcctcg cgtaaggtac ctatcgatag agaaatgttc tggcacctgc
      301 acttgcactg gggacagcct attttgctag tttgttttgt ttcgttttgt tttgatggag
      361 agcgtatgtt agtactatcg attcacacaa aaaaccaaca cacagatgta atgaaaataa
      421 agatatttta ttgcggccgc tccaagtacc tcccgtacct taatattact tacttatcat
      481 ggtagcttgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      541 cgcagcctga atggcgaatg gcaaattgta agcgttaata ttttgttaaa attcgcgtta
      601 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
      661 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
      721 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
      781 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
      841 aatcgga
//
