LOCUS       sequence-16396-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Monsalve M, Wu Z, Adelmant G, Puigserver P, Fan M, Spiegelman BM
  TITLE     Direct coupling of transcription and mRNA processing through the 
            thermogenic coactivator PGC-1.
  JOURNAL   Mol Cell 2000 Aug;6(2):307-16.
  PUBMED    10983978
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2000-08"; volume: "6(2)"; pages: "307-16"
COMMENT     Sequence Label: pcDNA-f:PGC1
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             164..187
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagcttggt accgagctcg gatccactag
      121 tccagtgtgg tggaattctg cagatatccg gatcaccacc atggactaca aagacgatga
      181 cgataaagca agaagagcat ctgtgggatc ctgcagccaa gactctgtat ggagtgacat
      241 agagtgtgct gctctggttg gtgaggacca gcctctttgc ccagatcttc ctgaacttga
      301 cctttctgaa cttgatgtga atgacttgga tacagacagc tttctgggtg gattgaagtg
      361 gtgtagcgac caatcggaaa tcatatccaa ccagtacaac aatgagcctg cgaacatatt
      421 tgagaagata gatgaagaga atgaggcaaa cttgctagcg gtcctcacag agacactgga
      481 cagtctcccc gtggatgaag acggattgcc ctcatttgat gcactgacag atggagccgt
      541 gaccactgac aacgaggcca gtccttcctc catgcctgac ggcacccctc cccctcagga
      601 ggcagaagag ccgtctctac ttaagaagct cttactggca ccagccaaca ctcagctcag
      661 ctacaatgaa tgcagcggtc ttagcactca gaaccatgca gcaaaccaca cccacaggat
      721 cagaacaaac cctgccattg ttaagaccga gaattcatgg agcaataaag cgaagagcat
      781 ttgtcaacag caaaagccac aaagacgtcc ctgctcagag cttctcaagt atctgaccac
      841 aaacgatgac cctcctcaca ccaaacccac agaaaacagg aacagcagca gagacaaatg
      901 tgcttccaaa aagaagtccc atacacaacc gcagtcgcaa catgctcaag ccaaaccaac
      961 aactttat
//
