LOCUS       sequence-163965-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV promoter driving expression of GinB catalytic domain fused with 
            linker to dCas9 in the order shown. Has a FLAG and NLS tag on the 
            c-terminus. Spec resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgctcattgg ctacgtgcgc gtctcaacta acgaccagaa
      121 taccgatctt cagaggaacg cactggtttg tgcaggctgc gaacagattt tcgaggacaa
      181 actcagcggg acacggacgg acagacctgg cctcaagcga gcactcaaga ggctgcagaa
      241 aggagacact ctggtggtct ggaaattgga ccgcctgggt cgaagcatga agcatctcat
      301 ttctctggtt ggcgaactgc gagaaagggg gatcaacttt cgaagtctga cggattccat
      361 agatacaagc agccccatgg gccggttctt cttctacgtg atgggtgcac tggctgaaat
      421 ggaaagagaa ctcattatag agcgaaccat ggcagggctt gcggctgcca ggaataaagg
      481 caggcggttt ggaagaccac caaagggtgg atccggaggg tccggaggta gtggcggcag
      541 cggtggttca ggtggcagcg gagggtcagg aggctctgat aaaaagtatt ctattggttt
      601 agctatcggc actaattccg ttggatgggc tgtcataacc gatgaataca aagtaccttc
      661 aaagaaattt aaggtgttgg ggaacacaga ccgtcattcg attaaaaaga atcttatcgg
      721 tgccctccta ttcgatagtg gcgaaacggc agaggcgact cgcctgaaac gaaccgctcg
      781 gagaaggtat acacgtcgca agaaccgaat atgttactta caagaaattt ttagcaatga
      841 gatggccaaa gttgacgat
//
