LOCUS       sequence-163966-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CMV promoter driving expression of GinB catalytic domain fused with 
            linker to dCas9 in the order shown. Has a FLAG and NLS tag on the 
            c-terminus. Spec resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..45)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(55..78)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgatggtga tgatgaccgg ttcagacctt ccttttcttc tttggggaac ctcccttgtc
       61 gtcatcatcc ttataatcgg agccaccgtc acccccaagc tgtgacaaat ctatccgagt
      121 ttcatataat cccgtgatgg attggtgaat cagtgtcgcg tctagcacct ccttggtaga
      181 agtgtatcgt ttgcgatcta tcgttgtgtc aaaatacttg aatgcggctg gagcgccgag
      241 gttggtaaga gtaaacaaat ggataatatt ttccgcctgc tcacgtatgg gtttatccct
      301 gtgcttgttg tatgcgctta atactttgtc cagattggca tcagctagga tgactctctt
      361 actgaattcc gaaatttgct ctatgatttc gtcgagataa tgtttgtgct gctcaacaaa
      421 aagttgcttc tgttcgttat cttcaggtga acctttcaac ttctcgtaat gggacgctaa
      481 atacaggaaa ttcacgtatt tagacggtag tgcgagttcg ttcccctttt gaagctctcc
      541 ggcgctagcc aacatccgtt ttcggccatt ttctaactca aacagactat actttggtag
      601 tttaattatg agatcctttt ttacttcctt gtaacctttc gcctcaagga agtcgatggg
      661 gttcttttca aaagacgagc gctccataat cgttatcccc aataattctt tgactgactt
      721 cagtttcttg gattttccct tctcaacttt tgc
//
