LOCUS       sequence-163975-         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized Cas9 for expression in S. cerevisiae 
            (budding yeast) from the TEF1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Dicarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM.
  TITLE     Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas 
            systems.
  JOURNAL   Nucleic Acids Res
  PUBMED    23460208
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"
COMMENT     Sequence Label: p414-TEF1p-Cas9-CYC1t
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..72
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     56..72
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 acttttttta cttcttgctc attagaaaga aagcatagca atctaatcta agttttctag
       61 aactagtgga tcccccggga aaaatggaca agaagtactc cattgggctc gatatcggca
      121 caaacagcgt cggttgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca
      181 aagttctggg caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt
      241 tcgactccgg ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata
      301 cccgcagaaa gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg
      361 tggatgactc tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc
      421 acgagcgcca cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc
      481 caaccatata tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt
      541 tgatctatct cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg
      601 acctgaaccc agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca
      661 atcagctttt cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga
      721 gcgctaggct gtccaaatcc
//
