LOCUS       sequence-163976-         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Protein Expression Vector , Excitation: 399, Emission:
            456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-pBAD
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..87
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             128..160
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             173..193
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttt gggctagaaa
       61 taattttgtt taactttaag aaggagatat acatatgcgg ggttctcatc atcatcatca
      121 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgagaacct
      181 gtacttccag ggctcgagca tggtgtctaa gggcgaagag ctgattaagg agaacatgca
      241 catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg
      301 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
      361 tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat
      421 caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg
      481 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
      541 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg
      601 ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc tgtaccccgc
      661 tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct
      721 gatcgcaaac gccaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc
      781 tg
//
