LOCUS       sequence-164002-        4026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SUV39H1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SUV39H1_WT
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     364..383
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     617..634
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(793..820)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(912..998)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1047..1069
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1061..1078
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1062..1078
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1094..1193
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2430..2529)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2546..2565)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2547..2565)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2570..2586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2570..2586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3017..3808
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3901..4026,1..463)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
       61 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      121 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      181 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      241 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      301 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      361 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      421 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      481 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      541 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      601 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      661 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
      721 ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc aggaagagtt
      781 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      841 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      901 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      961 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
     1021 cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga cggccagtct
     1081 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
     1141 aattgatgag caatgctttt ttataatgcc aactttgtac aaaaaagttg gcatggcgga
     1201 aaatttaaaa ggctgcagcg tgtgttgcaa gtcttcttgg aatcagctgc aggacctgtg
     1261 ccgcctggcc aagctctcct gccctgccct cggtatctct aagaggaacc tctatgactt
     1321 tgaagtcgag tacctgtgcg attacaagaa gatccgcgaa caggaatatt acctggtgaa
     1381 atggcgtgga tatccagact cagagagcac ctgggagcca cggcagaatc tcaagtgtgt
     1441 gcgtatcctc aagcagttcc acaaggactt agaaagggag ctgctccggc ggcaccaccg
     1501 gtcaaagacc ccccggcacc tggacccaag cttggccaac tacctggtgc agaaggccaa
     1561 gcagaggcgg gcgctccgtc gctgggagca ggagctcaat gccaagcgca gccatctggg
     1621 acgcatcact gtagagaatg aggtggacct ggacggccct ccgcgggcct tcgtgtacat
     1681 caatgagtac cgtgttggtg agggcatcac cctcaaccag gtggctgtgg gctgcgagtg
     1741 ccaggactgt ctgtgggcac ccactggagg ctgctgcccg ggggcgtcac tgcacaagtt
     1801 tgcctacaat gaccagggcc aggtgcggct tcgagccggg ctgcccatct acgagtgcaa
     1861 ctcccgctgc cgctgcggct atgactgccc aaatcgtgtg gtacagaagg gtatccgata
     1921 tgacctctgc atcttccgca cggatgatgg gcgtggctgg ggcgtccgca ccctggagaa
     1981 gattcgcaag aacagcttcg tcatggagta cgtgggagag atcattacct cagaggaggc
     2041 agagcggcgg ggccagatct acgaccgtca gggcgccacc tacctctttg acctggacta
     2101 cgtggaggac gtgtacaccg tggatgccgc ctactatggc aacatctccc actttgtcaa
     2161 ccacagttgt gaccccaacc tgcaggtgta caacgtcttc atagacaacc ttgacgagcg
     2221 gctgccccgc atcgctttct ttgccacaag aaccatccgg gcaggcgagg agctcacctt
     2281 tgattacaac atgcaagtgg accccgtgga catggagagc acccgcatgg actccaactt
     2341 tggcctggct gggctccctg gctcccctaa gaagcgggtc cgtattgaat gcaagtgtgg
     2401 gactgagtcc tgccgcaaat acctcttcta cccaactttc ttgtacaaag ttggcattat
     2461 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2521 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2581 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2641 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc
     2701 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
     2761 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     2821 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     2881 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     2941 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     3001 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     3061 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     3121 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     3181 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     3241 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     3301 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     3361 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     3421 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     3481 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     3541 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     3601 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     3661 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     3721 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     3781 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa
     3841 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3901 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3961 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4021 cagcag
//
