LOCUS       sequence-164003-        4068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TGFBR1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TGFBR1_WT
FEATURES             Location/Qualifiers
     source          1..4068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             115..906
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      999..1587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1488..1507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1741..1758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1917..1944)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2036..2122)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2171..2193
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2185..2202
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2186..2202
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2218..2317
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3596..3695)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3712..3731)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3713..3731)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3736..3752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3736..3752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
       61 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg
      121 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
      181 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
      241 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
      301 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
      361 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
      421 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
      481 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
      541 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
      601 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
      661 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
      721 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
      781 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
      841 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
      901 aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
      961 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1021 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1081 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1141 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1201 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1261 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1321 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1381 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1441 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1501 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1561 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1621 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1681 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1741 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     1801 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     1861 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
     1921 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
     1981 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
     2041 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
     2101 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
     2161 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
     2221 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
     2281 ttttttataa tgccaacttt gtacaaaaaa gttggcatgg aggcggcggt cgctgctccg
     2341 cgtccccggc tgctcctcct cgtgctggcg gcggcggcgg cggcggcggc ggcgctgctc
     2401 ccgggggcga cggcgttaca gtgtttctgc cacctctgta caaaagacaa ttttacttgt
     2461 gtgacagatg ggctctgctt tgtctctgtc acagagacca cagacaaagt tatacacaac
     2521 agcatgtgta tagctgaaat tgacttaatt cctcgagata ggccgtttgt atgtgcaccc
     2581 tcttcaaaaa ctgggtctgt gactacaaca tattgctgca atcaggacca ttgcaataaa
     2641 atagaacttc caactactgg tttaccattg cttgttcaga gaacaattgc gagaactatt
     2701 gtgttacaag aaagcattgg caaaggtcga tttggagaag tttggagagg aaagtggcgg
     2761 ggagaagaag ttgctgttaa gatattctcc tctagagaag aacgttcgtg gttccgtgag
     2821 gcagagattt atcaaactgt aatgttacgt catgaaaaca tcctgggatt tatagcagca
     2881 gacaataaag acaatggtac ttggactcag ctctggttgg tgtcagatta tcatgagcat
     2941 ggatcccttt ttgattactt aaacagatac acagttactg tggaaggaat gataaaactt
     3001 gctctgtcca cggcgagcgg tcttgcccat cttcacatgg agattgttgg tacccaagga
     3061 aagccagcca ttgctcatag agatttgaaa tcaaagaata tcttggtaaa gaagaatgga
     3121 acttgctgta ttgcagactt aggactggca gtaagacatg attcagccac agataccatt
     3181 gatattgctc caaaccacag agtgggaaca aaaaggtaca tggcccctga agttctcgat
     3241 gattccataa atatgaaaca ttttgaatcc ttcaaacgtg ctgacatcta tgcaatgggc
     3301 ttagtattct gggaaattgc tcgacgatgt tccattggtg gaattcatga agattaccaa
     3361 ctgccttatt atgatcttgt accttctgac ccatcagttg aagaaatgag aaaagttgtt
     3421 tgtgaacaga agttaaggcc aaatatccca aacagatggc agagctgtga agccttgaga
     3481 gtaatggcta aaattatgag agaatgttgg tatgccaatg gagcagctag gcttacagca
     3541 ttgcggatta agaaaacatt atcgcaactc agtcaacagg aaggcatcaa aatgtaccca
     3601 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     3661 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     3721 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     3781 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     3841 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     3901 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     3961 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     4021 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtct
//
