LOCUS       sequence-164004-        5163 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TNFAIP3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TNFAIP3_WT
FEATURES             Location/Qualifiers
     source          1..5163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      505..1093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     994..1013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1247..1264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1423..1450)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1542..1628)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1677..1699
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1691..1708
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1692..1708
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1724..1823
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4197..4296)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4313..4332)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4314..4332)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4337..4353)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4337..4353)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(4784..5163,1..412)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
       61 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
      121 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
      181 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
      241 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
      301 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
      361 agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag
      421 ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
      481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1201 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1261 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1321 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1381 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
     1441 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
     1501 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
     1561 gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt
     1621 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
     1681 gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa tgattttatt
     1741 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
     1801 aactttgtac aaaaaagttg gcaccatggc tgaacaagtc cttcctcagg ctttgtattt
     1861 gagcaatatg cggaaagctg tgaagatacg ggagagaact ccagaagaca tttttaaacc
     1921 tactaatggg atcattcatc attttaaaac catgcaccga tacacactgg aaatgttcag
     1981 aacttgccag ttttgtcctc agtttcggga gatcatccac aaagccctca tcgacagaaa
     2041 catccaggcc accctggaaa gccagaagaa actcaactgg tgtcgagaag tccggaagct
     2101 tgtggcgctg aaaacgaacg gtgacggcaa ttgcctcatg catgccactt ctcagtacat
     2161 gtggggcgtt caggacacag acttggtact gaggaaggcg ctgttcagca cgctcaagga
     2221 aacagacaca cgcaacttta aattccgctg gcaactggag tctctcaaat ctcaggaatt
     2281 tgttgaaacg gggctttgct atgatactcg gaactggaat gatgaatggg acaatcttat
     2341 caaaatggct tccacagaca cacccatggc ccgaagtgga cttcagtaca actcactgga
     2401 agaaatacac atatttgtcc tttgcaacat cctcagaagg ccaatcattg tcatttcaga
     2461 caaaatgcta agaagtttgg aatcaggttc caatttcgcc cctttgaaag tgggtggaat
     2521 ttacttgcct ctccactggc ctgcccagga atgctacaga taccccattg ttctcggcta
     2581 tgacagccat cattttgtac ccttggtgac cctgaaggac agtgggcctg aaatccgagc
     2641 tgttccactt gttaacagag accggggaag atttgaagac ttaaaagttc actttttgac
     2701 agatcctgaa aatgagatga aggagaagct cttaaaagag tacttaatgg tgatagaaat
     2761 ccccgtccaa ggctgggacc atggcacaac tcatctcatc aatgccgcaa agttggatga
     2821 agctaactta ccaaaagaaa tcaatctggt agatgattac tttgaacttg ttcagcatga
     2881 gtacaagaaa tggcaggaaa acagcgagca ggggaggaga gaggggcacg cccagaatcc
     2941 catggaacct tccgtgcccc agctttctct catggatgta aaatgtgaaa cgcccaactg
     3001 ccccttcttc atgtctgtga acacccagcc tttatgccat gagtgctcag agaggcggca
     3061 aaagaatcaa aacaaactcc caaagctgaa ctccaagccg ggccctgagg ggctccctgg
     3121 catggcgctc ggggcctctc ggggagaagc ctatgagccc ttggcgtgga accctgagga
     3181 gtccactggg gggcctcatt cggccccacc gacagcaccc agcccttttc tgttcagtga
     3241 gaccactgcc atgaagtgca ggagccccgg ctgccccttc acactgaatg tgcagcacaa
     3301 cggattttgt gaacgttgcc acaacgcccg gcaacttcac gccagccacg ccccagacca
     3361 cacaaggcac ttggatcccg ggaagtgcca agcctgcctc caggatgtta ccaggacatt
     3421 taatgggatc tgcagtactt gcttcaaaag gactacagca gaggcctcct ccagcctcag
     3481 caccagcctc cctccttcct gtcaccagcg ttccaagtca gatccctcgc ggctcgtccg
     3541 gagcccctcc ccgcattctt gccacagagc tggaaacgac gcccctgctg gctgcctgtc
     3601 tcaagctgca cggactcctg gggacaggac ggggacgagc aagtgcagaa aagccggctg
     3661 cgtgtatttt gggactccag aaaacaaggg cttttgcaca ctgtgtttca tcgagtacag
     3721 agaaaacaaa cattttgctg ctgcctcagg gaaagtcagt cccacagcgt ccaggttcca
     3781 gaacaccatt ccgtgcctgg ggagggaatg cggcaccctt ggaagcacca tgtttgaagg
     3841 atactgccag aagtgtttca ttgaagctca gaatcagaga tttcatgagg ccaaaaggac
     3901 agaagagcaa ctgagatcga gccagcgcag agatgtgcct cgaaccacac aaagcacctc
     3961 aaggcccaag tgcgcccggg cctcctgcaa gaacatcctg gcctgccgca gcgaggagct
     4021 ctgcatggag tgtcagcatc ccaaccagag gatgggccct ggggcccacc ggggtgagcc
     4081 tgcccccgaa gaccccccca agcagcgttg ccgggccccc gcctgtgatc attttggcaa
     4141 tgccaagtgc aacggctact gcaacgaatg ctttcagttc aagcagatgt atggcttgcc
     4201 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     4261 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     4321 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     4381 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     4441 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     4501 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     4561 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     4621 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     4681 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     4741 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     4801 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     4861 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     4921 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     4981 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     5041 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     5101 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     5161 gat
//
