LOCUS       sequence-164006-        3930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing WNT5A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WNT5A_WT
FEATURES             Location/Qualifiers
     source          1..3930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             347..1138
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1231..1819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1720..1739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1973..1990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2149..2176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2268..2354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2403..2425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2417..2434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2418..2434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2450..2549
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3690..3789)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3806..3825)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3807..3825)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3830..3846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3830..3846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggacagaaat gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa
       61 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
      121 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
      181 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
      241 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
      301 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt
      361 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
      421 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
      481 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
      541 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
      601 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
      661 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
      721 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
      781 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
      841 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
      901 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
      961 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     1021 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     1081 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc
     1141 ctcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     1201 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1261 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1321 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1381 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1441 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1501 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1561 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1621 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1681 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     1801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     1861 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     1921 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     1981 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2041 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2101 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
     2161 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
     2221 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
     2281 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc
     2341 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
     2401 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc aaataatgat
     2461 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
     2521 aatgccaact ttgtacaaaa aagttggcat gaagaagtcc attggaatat taagcccagg
     2581 agttgctttg gggatggctg gaagtgcaat gtcttccaag ttcttcctag tggctttggc
     2641 catatttttc tccttcgccc aggttgtaat tgaagccaat tcttggtggt cgctaggtat
     2701 gaataaccct gttcagatgt cagaagtata tattatagga gcacagcctc tctgcagcca
     2761 actggcagga ctttctcaag gacagaagaa actgtgccac ttgtatcagg accacatgca
     2821 gtacatcgga gaaggcgcga agacaggcat caaagaatgc cagtatcaat tccgacatcg
     2881 aaggtggaac tgcagcactg tggataacac ctctgttttt ggcagggtga tgcagatagg
     2941 cagccgcgag acggccttca catacgcggt gagcgcagca ggggtggtga acgccatgag
     3001 ccgggcgtgc cgcgagggcg agctgtccac ctgcggctgc agccgcgccg cgcgccccaa
     3061 ggacctgccg cgggactggc tctggggcgg ctgcggcgac aacatcgact atggctaccg
     3121 ctttgccaag gagttcgtgg acgcccgcga gcgggagcgc atccacgcca agggctccta
     3181 cgagagtgct cgcatcctca tgaacctgca caacaacgag gccggccgca ggacggtgta
     3241 caacctggct gatgtggcct gcaagtgcca tggggtgtcc ggctcatgta gcctgaagac
     3301 atgctggctg cagctggcag acttccgcaa ggtgggtgat gccctgaagg agaagtacga
     3361 cagcgcggcg gccatgcggc tcaacagccg gggcaagttg gtacaggtca acagccgctt
     3421 caactcgccc accacacaag acctggtcta catcgacccc agccctgact actgcgtgcg
     3481 caatgagagc accggctcgc tgggcacgca gggccgcctg tgcaacaaga cgtcggaggg
     3541 catggatggc tgcgagctca tgtgctgcgg ccgtggctac gaccagttca agaccgtgca
     3601 gacggagcgc tgccactgca agttccactg gtgctgctac gtcaagtgca agaagtgcac
     3661 ggagatcgtg gaccagtttg tgtgcaagta cccaactttc ttgtacaaag ttggcattat
     3721 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     3781 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     3841 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     3901 tatatcatca tgcctcctct agaccagcca
//
