LOCUS       sequence-164007-        3633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing GEMIN2 ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SIP1_WT
FEATURES             Location/Qualifiers
     source          1..3633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..247
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     481..498
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(657..684)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(776..862)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     911..933
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     925..942
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     926..942
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    958..1057
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1901..2000)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2017..2036)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2018..2036)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2041..2057)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2041..2057)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2488..3279
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3372..3633,1..327)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
       61 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      121 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      181 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      241 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      301 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      361 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      421 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      481 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      541 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      601 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
      661 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
      721 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
      781 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg
      841 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
      901 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa
      961 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
     1021 ttttttataa tgccaacttt gtacaaaaaa gttggcacca tgcgccgagc ggaactggct
     1081 ggtttgaaaa ccatggcgtg ggtaccagcg gagtccgcag tggaagagtt gatgcctcgg
     1141 ctattgccgg tagagccttg cgacttgacg gaaggtttcg atccctcggt acccccgagg
     1201 acgcctcagg aatacctgag gcgggtccag atcgaagcag ctcaatgtcc agatgttgtg
     1261 gtagctcaaa ttgacccaaa gaagttgaaa aggaagcaaa gtgtgaatat ttctctttca
     1321 ggatgccaac ccgcccctga aggttattcc ccaacacttc aatggcaaca gcaacaagtg
     1381 gcacagtttt caactgttcg acagaatgtg aacaaacata gaagtcactg gaaatcacaa
     1441 cagttggata gtaatgtgac aatgccaaaa tctgaagatg aagaaggctg gaagaaattt
     1501 tgtctgggtg aaaagttatg tgctgacggg gctgttggac cagccacaaa tgaaagtcct
     1561 ggaatagatt atgtacaaat tggttttcct cccttgctta gtattgttag cagaatgaat
     1621 caggcaacag taactagtgt cttggaatat ctgagtaatt ggtttggaga aagagacttt
     1681 actccagaat tgggaagatg gctttatgct ttattggctt gtcttgaaaa gcctttgtta
     1741 cctgaggctc attcactgat tcggcagctt gcaagaaggt gctctgaagt gaggctctta
     1801 gtggatagca aagatgatga gagggttcct gctttgaatt tattaatctg cttggttagc
     1861 aggtattttg accaacgtga tttagctgat gagccatctt tgccaacttt cttgtacaaa
     1921 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     1981 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     2041 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     2101 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     2161 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     2221 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     2281 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     2341 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     2401 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     2461 agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact
     2521 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     2581 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     2641 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     2701 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2761 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2821 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2881 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2941 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     3001 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     3061 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     3121 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     3181 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     3241 tacgtgaaag gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca
     3301 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3481 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3601 ccagtggctg ctgccagtgg cgataagtcg tgt
//
