LOCUS       sequence-164011-        4047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing IKBKG , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IKBKG_WT
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     495..514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     748..765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(924..951)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1043..1129)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1178..1200
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1192..1209
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1193..1209
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1225..1324
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2582..2681)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2698..2717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2699..2717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2722..2738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2722..2738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3169..3960
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
       61 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
      601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
      661 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
      721 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      781 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      841 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      901 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      961 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     1021 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     1081 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     1141 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     1201 acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt
     1261 tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagtt
     1321 ggcatgaata ggcacctctg gaagagccaa ctgtgtgaga tggtgcagcc cagtggtggc
     1381 ccggcagcag atcaggacgt actgggcgaa gagtctcctc tggggaagcc agccatgctg
     1441 cacctgcctt cagaacaggg cgctcctgag accctccagc gctgcctgga ggagaatcaa
     1501 gagctccgag atgccatccg gcagagcaac cagattctgc gggagcgctg cgaggagctt
     1561 ctgcatttcc aagccagcca gagggaggag aaggagttcc tcatgtgcaa gttccaggag
     1621 gccaggaaac tggtggagag actcggcctg gagaagctcg atctgaagag gcagaaggag
     1681 caggctctgc gggaggtgga gcacctgaag agatgccagc agcagatggc tgaggacaag
     1741 gcctctgtga aagcccaggt gacgtccttg ctcggggagc tgcaggagag ccagagtcgc
     1801 ttggaggctg ccactaagga atgccaggct ctggagggtc gggcccgggc ggccagcgag
     1861 caggcgcggc agctggagag tgagcgcgag gcgctgcagc agcagcacag cgtgcaggtg
     1921 gaccagctgc gcatgcaggg ccagagcgtg gaggccgcgc tccgcatgga gcgccaggcc
     1981 gcctcggagg agaagaggaa gctggcccag ttgcaggtgg cctatcacca gctcttccaa
     2041 gaatacgaca accacatcaa gagcagcgtg gtgggcagtg agcggaagcg aggaatgcag
     2101 ctggaagatc tcaaacagca gctccagcag gccgaggagg ccctggtggc caaacaggag
     2161 gtgatcgata agctgaagga ggaggccgag cagcacaaga ttgtgatgga gaccgttccg
     2221 gtgctgaagg cccaggcgga tatctacaag gcggacttcc aggctgagag gcaggcccgg
     2281 gagaagctgg ccgagaagaa ggagctcctg caggagcagc tggagcagct gcagagggag
     2341 tacagcaaac tgaaggccag ctgtcaggag tcggccagga tcgaggacat gaggaagcgg
     2401 catgtcgagg tctcccaggc ccccttgccc cccgcccctg cctacctctc ctctcccctg
     2461 gccctgccca gccagaggag gagccccccc gaggagccac ctgacttctg ctgtcccaag
     2521 tgccagtatc aggcccctga tatggacacc ctgcagatac atgtcatgga gtgcattgag
     2581 tacccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc
     2641 aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc
     2701 tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa
     2761 aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct ctagaccagc
     2821 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     2881 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     2941 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     3001 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     3061 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     3121 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg
     3181 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     3241 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     3301 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     3361 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     3421 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     3481 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     3541 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     3601 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     3661 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     3721 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     3781 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     3841 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     3901 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     3961 ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     4021 tcagaccccg tagaaaagat caaagga
//
