LOCUS       sequence-164017-        4605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BTRC , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BTRC_WT
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      514..1102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1003..1022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1256..1273
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1432..1459)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1551..1637)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1686..1708
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1700..1717
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1701..1717
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1733..1832
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3648..3747)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3764..3783)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3765..3783)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3788..3804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3788..3804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(4235..4605,1..421)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
       61 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      121 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      181 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      241 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      301 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
      361 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
      421 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
      481 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      541 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      601 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      661 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      901 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1021 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1081 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1141 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1201 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     1261 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     1321 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     1381 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
     1441 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
     1501 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     1561 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
     1621 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
     1681 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat
     1741 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
     1801 tataatgcca actttgtaca aaaaagttgg catggacccg gccgaggcgg tgctgcaaga
     1861 gaaggcactc aagtttatgt gctctatgcc caggtctctg tggctgggct gctccagcct
     1921 ggcggacagc atgccttcgc tgcgatgcct gtataaccca gggactggcg cactcacagc
     1981 tttccagaat tcctcagaga gagaagactg taataatggc gaacccccta ggaagataat
     2041 accagagaag aattcactta gacagacata caacagctgt gccagactct gcttaaacca
     2101 agaaacagta tgtttagcaa gcactgctat gaagactgag aattgtgtgg ccaaaacaaa
     2161 acttgccaat ggcacttcca gtatgattgt gcccaagcaa cggaaactct cagcaagcta
     2221 tgaaaaggaa aaggaactgt gtgtcaaata ctttgagcag tggtcagagt cagatcaagt
     2281 ggaatttgtg gaacatctta tatcccaaat gtgtcattac caacatgggc acataaactc
     2341 gtatcttaaa cctatgttgc agagagattt cataactgct ctgccagctc ggggattgga
     2401 tcatattgct gagaacattc tgtcatacct ggatgccaaa tcactatgtg ctgctgaact
     2461 tgtgtgcaag gaatggtacc gagtgacctc tgatggcatg ctgtggaaga agcttatcga
     2521 gagaatggtc aggacagatt ctctgtggag aggcctggca gaacgaagag gatggggaca
     2581 gtatttattc aaaaacaaac ctcctgacgg gaatgctcct cccaactctt tttatagagc
     2641 actttatcct aaaattatac aagacattga gacaatagaa tctaattgga gatgtggaag
     2701 acatagttta cagagaattc actgccgaag tgaaacaagc aaaggagttt actgtttaca
     2761 gtatgatgat cagaaaatag taagcggcct tcgagacaac acaatcaaga tctgggataa
     2821 aaacacattg gaatgcaagc gaattctcac aggccataca ggttcagtcc tctgtctcca
     2881 gtatgatgag agagtgatca taacaggatc atcggattcc acggtcagag tgtgggatgt
     2941 aaatacaggt gaaatgctaa acacgttgat tcaccattgt gaagcagttc tgcacttgcg
     3001 tttcaataat ggcatgatgg tgacctgctc caaagatcgt tccattgctg tatgggatat
     3061 ggcctcccca actgacatta ccctccggag ggtgctggtc ggacaccgag ctgctgtcaa
     3121 tgttgtagac tttgatgaca agtacattgt ttctgcatct ggggatagaa ctataaaggt
     3181 atggaacaca agtacttgtg aatttgtaag gaccttaaat ggacacaaac gaggcattgc
     3241 ctgtttgcag tacagggaca ggctggtagt gagtggctca tctgacaaca ctatcagatt
     3301 atgggacata gaatgtggtg catgtttacg agtgttagaa ggccatgagg aattggtgcg
     3361 ttgtattcga tttgataaca agaggatagt cagtggggcc tatgatggaa aaattaaagt
     3421 gtgggatctt gtggctgctt tggacccccg tgctcctgca gggacactct gtctacggac
     3481 ccttgtggag cattccggaa gagtttttcg actacagttt gatgaattcc agattgtcag
     3541 tagttcacat gatgacacaa tcctcatctg ggacttccta aatgatccag ctgcccaagc
     3601 tgaacccccc cgttcccctt ctcgaacata cacctacatc tccagatacc caactttctt
     3661 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     3721 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     3781 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     3841 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     3901 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     3961 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     4021 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     4081 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     4141 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     4201 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     4261 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     4321 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     4381 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     4441 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     4501 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     4561 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagcc
//
