LOCUS       sequence-164018-        3465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing SOCS3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_SOCS3_WT
FEATURES             Location/Qualifiers
     source          1..3465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     463..482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     716..733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(892..919)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1011..1097)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1146..1168
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1160..1177
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1161..1177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1193..1292
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1968..2067)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2084..2103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2085..2103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2108..2124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2108..2124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2555..3346
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3439..3465,1..562)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
       61 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
      121 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
      181 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
      241 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
      301 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
      361 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      421 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      481 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      541 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      601 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      661 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      721 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      781 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      841 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
      901 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
      961 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     1021 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
     1081 gcctttcgtt ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat
     1141 gttttcccag tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cccaaataat
     1201 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
     1261 tataatgcca actttgtaca aaaaagttgg catggtcacc cacagcaagt ttcccgccgc
     1321 cgggatgagc cgccccctgg acaccagcct gcgcctcaag accttcagct ccaagagcga
     1381 gtaccagctg gtggtgaacg cagtgcgcaa gctgcaggag agcggcttct actggagcgc
     1441 agtgaccggc ggcgaggcga acctgctgct cagtgccgag cccgccggca cctttctgat
     1501 ccgcgacagc tcggaccagc gccacttctt cacgctcagc gtcaagaccc agtctgggac
     1561 caagaacctg cgcatccagt gtgagggggg cagcttctct ctgcagagcg atccccggag
     1621 cacgcagccc gtgccccgct tcgactgcgt gctcaagctg gtgcaccact acatgccgcc
     1681 ccctggagcc ccctccttcc cctcgccacc tactgaaccc tcctccgagg tgcccgagca
     1741 gccgtctgcc cagccactcc ctgggagtcc ccccagaaga gcctattaca tctactccgg
     1801 gggcgagaag atccccctgg tgttgagccg gcccctctcc tccaacgtgg ccactcttca
     1861 gcatctctgt cggaagaccg tcaacggcca cctggactcc tatgagaaag tcacccagct
     1921 gccggggccc attcgggagt tcctggacca gtacgatgcc ccgctttacc caactttctt
     1981 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2041 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2101 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2161 cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg acagaaatgc
     2221 ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc
     2281 acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg
     2341 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     2401 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     2461 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     2521 gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga tcgccgaagt
     2581 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     2641 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     2701 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     2761 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     2821 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     2881 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     2941 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     3001 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     3061 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     3121 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     3181 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     3241 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     3301 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct cgagccaccc
     3361 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     3421 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaat
//
