LOCUS       sequence-164021-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP- NleG6-3 EHEC in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub69-NleG6-3 EHEC
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             80..91
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             464..481
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac atcagcagga
       61 cgcactgacc gaattcatta aagaggagaa aacgcgtatg agtaaagtag acatggtttg
      121 gatagtcgga ggcagttctg tttaccagga agccatgaat caaccaggcc acctcagact
      181 ctttgtgaca aggatcatgc aggaatttga aagtgacacg tttttcccag aaattgattt
      241 ggggaaatat aaacttctcc cagaataccc aggcgtcctc tctgaggtcc aggaggaaaa
      301 aggcatcaag tataagtttg aagtctacga gaagaaagac aggcgcgccg gcggctcagc
      361 gggctcggga agcggagcgg gatctggcag cggcggtagc gcaggcagca gcgggtcaag
      421 cggtgcctcg agcggcaggc gcgccctgca gatgggcagc agccatcacc atcatcacca
      481 cagccaggat ccgaattcga aaatcgaaga aggtaaactg gtaatctgga ttaacggcga
      541 taaaggctat aacggtctcg ctgaagtcgg taagaaattc gagaaagata ccggaattaa
      601 agtcaccgtt gagcatccgg ataaactgga agagaaattc ccacaggttg cggcaactgg
      661 cgatggccct gacattatct tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg
      721 cctgttggct gaaatcaccc cggacaaagc gttccaggac aagctgtatc cgtttacctg
      781 ggatgccgta cgttacaacg gcaagctgat tgcttacccg atcgctgttg aagcgttatc
      841 gctgatttat aacaaagatc tgctgccgaa cccgccaaaa acctgggaag agatcccggc
      901 gctggataaa gaactgaaag cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc
      961 gtacttcacc tggccgctga ttgctgctga cgggggttat gcgttcaagt atgaaaacgg
     1021 ca
//
