LOCUS       sequence-164046-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP-Sem1 Sc in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub100-Sem1 Sc
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             74..85
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             362..379
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc aggacgcact
       61 gaccgaattc attaaagagg agaaaacgcg tatgagtaaa gtagacatgg tttggatagt
      121 cggaggcagt tctgtttacc aggaagccat gaatcaacca ggccacctca gactctttgt
      181 gacaaggatc atgcaggaat ttgaaagtga cacgtttttc ccagaaattg atttggggaa
      241 atataaactt ctcccagaat acccaggcgt cctctctgag gtccaggagg aaaaaggcat
      301 caagtataag tttgaagtct acgagaagaa agacaggcgc gccctgcaga tgggcagcag
      361 ccatcaccat catcaccaca gccaggatcc gaattcgaaa atcgaagaag gtaaactggt
      421 aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga
      481 gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc
      541 acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg
      601 tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa
      661 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat
      721 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac
      781 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaa
//
