LOCUS       sequence-164047-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the cDHFR-link2-His6-MBP-Sem1 Sc in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Levin-Kravets O, Tanner N, Shohat N, Attali I, Keren-Kaplan T, 
            Shusterman A, Artzi S, Varvak A, Reshef Y, Shi X, Zucker O, Baram T,
            Katina C, Pilzer I, Ben-Aroya S, Prag G
  TITLE     A bacterial genetic selection system for ubiquitylation cascade 
            discovery.
  JOURNAL   Nat Methods. 2016 Nov;13(11):945-952. doi: 10.1038/nmeth.4003. Epub 
            2016 Oct 3.
  PUBMED    27694912
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4003"; journalName: "Nat Methods"; date: "2016-11"; 
            volume: "13"; issue: "11"; pages: "945-952"
COMMENT     Sequence Label: pCD-Sub100-Sem1 Sc
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..52
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             286..297
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 taacaacaac ctcgggatcg atacgaccct ggaagttctg ttccaggggc ccccgcggat
       61 gagtacggac gttgctgctg cacaagccca aagtaaaata gatttaacaa aaaaaaaaaa
      121 tgaggaaata aataagaagt ctctagagga agacgatgaa tttgaagatt tccccattga
      181 cacatgggcc aatggtgaaa caataaaaag caatgctgta acgcaaacaa acatatggga
      241 agaaaactgg gatgatgttg aagttgacga cgatttcacc aatgaattga aggccgaatt
      301 ggatagatac aaacgggaaa atcagtgaac tagttaagtc gaacagaaag taatcgtatt
      361 gtacacggcc gaagcttggc gccatagcgg ccgcgagaag cacacggtca cactgcttcc
      421 ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc
      481 tgaaccgacg accgggtcat cgtggccgga tcttgcggcc cctcggcttg aacgaattgt
      541 tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
      601 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
      661 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
      721 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
      781 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
      841 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
      901 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
      961 cgtggctggc tcgaa
//
