LOCUS       sequence-164049-        5208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing FGFR3 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_FGFR3_K650E
FEATURES             Location/Qualifiers
     source          1..5208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2608..2707)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2724..2743)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2725..2743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2748..2764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2748..2764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3195..3986
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4079..4667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4568..4587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4821..4838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4997..5024)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5116..5202)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gggcgcccct gcctgcgccc tcgcgctctg cgtggccgtg gccatcgtgg
      241 ccggcgcctc ctcggagtcc ttggggacgg agcagcgcgt cgtggggcga gcggcagaag
      301 tcccgggccc agagcccggc cagcaggagc agttggtctt cggcagcggg gatgctgtgg
      361 agctgagctg tcccccgccc gggggtggtc ccatggggcc cactgtctgg gtcaaggatg
      421 gcacagggct ggtgccctcg gagcgtgtcc tggtggggcc ccagcggctg caggtgctga
      481 atgcctccca cgaggactcc ggggcctaca gctgccggca gcggctcacg cagcgcgtac
      541 tgtgccactt cagtgtgcgg gtgacagacg ctccatcctc gggagatgac gaagacgggg
      601 aggacgaggc tgaggacaca ggtgtggaca caggggcccc ttactggaca cggcccgagc
      661 ggatggacaa gaagctgctg gccgtgccgg ccgccaacac cgtccgcttc cgctgcccag
      721 ccgctggcaa ccccactccc tccatctcct ggctgaagaa cggcagggag ttccgcggcg
      781 agcaccgcat tggaggcatc aagctgcggc atcagcagtg gagcctggtc atggaaagcg
      841 tggtgccctc ggaccgcggc aactacacct gcgtcgtgga gaacaagttt ggcagcatcc
      901 ggcagacgta cacgctggac gtgctggagc gctccccgca ccggcccatc ctgcaggcgg
      961 ggctgccggc caaccagacg gcggtgctgg gcagcgacgt ggagttccac tgcaaggtgt
     1021 acagtgacgc acagccccac atccagtggc tcaagcacgt ggaggtgaat ggcagcaagg
     1081 tgggcccgga cggcacaccc tacgttaccg tgctcaagac ggcgggcgct aacaccaccg
     1141 acaaggagct agaggttctc tccttgcaca acgtcacctt tgaggacgcc ggggagtaca
     1201 cctgcctggc gggcaattct attgggtttt ctcatcactc tgcgtggctg gtggtgctgc
     1261 cagccgagga ggagctggtg gaggctgacg aggcgggcag tgtgtatgca ggcatcctca
     1321 gctacggggt gggcttcttc ctgttcatcc tggtggtggc ggctgtgacg ctctgccgcc
     1381 tgcgcagccc ccccaagaaa ggcctgggct cccccaccgt gcacaagatc tcccgcttcc
     1441 cgctcaagcg acaggtgtcc ctggagtcca acgcgtccat gagctccaac acaccactgg
     1501 tgcgcatcgc aaggctgtcc tcaggggagg gccccacgct ggccaatgtc tccgagctcg
     1561 agctgcctgc cgaccccaaa tgggagctgt ctcgggcccg gctgaccctg ggcaagcccc
     1621 ttggggaggg ctgcttcggc caggtggtca tggcggaggc catcggcatt gacaaggacc
     1681 gggccgccaa gcctgtcacc gtagccgtga agatgctgaa agacgatgcc actgacaagg
     1741 acctgtcgga cctggtgtct gagatggaga tgatgaagat gatcgggaaa cacaaaaaca
     1801 tcatcaacct gctgggcgcc tgcacgcagg gcgggcccct gtacgtgctg gtggagtacg
     1861 cggccaaggg taacctgcgg gagtttctgc gggcgcggcg gcccccgggc ctggactact
     1921 ccttcgacac ctgcaagccg cccgaggagc agctcacctt caaggacctg gtgtcctgtg
     1981 cctaccaggt ggcccggggc atggagtact tggcctccca gaagtgcatc cacagggacc
     2041 tggctgcccg caatgtgctg gtgaccgagg acaacgtgat gaagatcgca gacttcgggc
     2101 tggcccggga cgtgcacaac ctcgactact acaaggagac aaccaacggc cggctgcccg
     2161 tgaagtggat ggcgcctgag gccttgtttg accgagtcta cactcaccag agtgacgtct
     2221 ggtcctttgg ggtcctgctc tgggagatct tcacgctggg gggctccccg taccccggca
     2281 tccctgtgga ggagctcttc aagctgctga aggagggcca ccgcatggac aagcccgcca
     2341 actgcacaca cgacctgtac atgatcatgc gggagtgctg gcatgccgcg ccctcccaga
     2401 ggcccacctt caagcagctg gtggaggacc tggaccgtgt ccttaccgtg acgtccaccg
     2461 acgagtacct ggacctgtca gcgcctttcg agcagtactc cccgggtggc caggacaccc
     2521 ccagctccag ctcctcaggg gacgactccg tgtttgccca cgacctgctg cccccggccc
     2581 cacccagcag tgggggctcg cggacgtgac caactttctt gtacaaagtt ggcattataa
     2641 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2701 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     2761 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2821 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2881 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2941 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     3001 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     3061 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     3121 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3181 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     3241 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     3301 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3361 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     3421 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     3481 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3541 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3601 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3661 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     3721 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3781 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3841 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3901 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3961 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     4021 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4081 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4141 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4201 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4261 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4321 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4381 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4441 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4501 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4561 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4621 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4681 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4741 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4801 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4861 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4921 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4981 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     5041 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     5101 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     5161 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
