LOCUS       sequence-164050-        4644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BIRC2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BIRC2_WT
FEATURES             Location/Qualifiers
     source          1..4644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     350..369
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     603..620
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(779..806)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(898..984)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1033..1055
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1047..1064
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1048..1064
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1080..1179
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3034..3133)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3150..3169)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3151..3169)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3174..3190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3174..3190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3621..4412
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4505..4644,1..449)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
       61 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      121 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      181 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      241 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      301 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      361 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      421 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      481 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      541 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
      601 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
      661 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      721 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
      781 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
      841 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
      901 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
      961 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     1021 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc
     1081 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat
     1141 gcttttttat aatgccaact ttgtacaaaa aagttggcat gcacaaaact gcctcccaaa
     1201 gacttttccc aggtccctcg tatcaaaaca ttaagagtat aatggaagat agcacgatct
     1261 tgtcagattg gacaaacagc aacaaacaaa aaatgaagta tgacttttcc tgtgaactct
     1321 acagaatgtc tacatattca actttccccg ccggggtgcc tgtctcagaa aggagtcttg
     1381 ctcgtgctgg tttttattat actggtgtga atgacaaggt caaatgcttc tgttgtggcc
     1441 tgatgctgga taactggaaa ctaggagaca gtcctattca aaagcataaa cagctatatc
     1501 ctagctgtag ctttattcag aatctggttt cagctagtct gggatccacc tctaagaata
     1561 cgtctccaat gagaaacagt tttgcacatt cattatctcc caccttggaa catagtagct
     1621 tgttcagtgg ttcttactcc agcctttctc caaaccctct taattctaga gcagttgaag
     1681 acatctcttc atcgaggact aacccctaca gttatgcaat gagtactgaa gaagccagat
     1741 ttcttaccta ccatatgtgg ccattaactt ttttgtcacc atcagaattg gcaagagctg
     1801 gtttttatta tataggacct ggagataggg tagcctgctt tgcctgtggt gggaagctca
     1861 gtaactggga accaaaggat gatgctatgt cagaacaccg gaggcatttt cccaactgtc
     1921 catttttgga aaattctcta gaaactctga ggtttagcat ttcaaatctg agcatgcaga
     1981 cacatgcagc tcgaatgaga acatttatgt actggccatc tagtgttcca gttcagcctg
     2041 agcagcttgc aagtgctggt ttttattatg tgggtcgcaa tgatgatgtc aaatgctttt
     2101 gttgtgatgg tggcttgagg tgttgggaat ctggagatga tccatgggta gaacatgcca
     2161 agtggtttcc aaggtgtgag ttcttgatac gaatgaaagg ccaagagttt gttgatgaga
     2221 ttcaaggtag atatcctcat cttcttgaac agctgttgtc aacttcagat accactggag
     2281 aagaaaatgc tgacccacca attattcatt ttggacctgg agaaagttct tcagaagatg
     2341 ctgtcatgat gaatacacct gtggttaaat ctgccttgga aatgggcttt aatagagacc
     2401 tggtgaaaca aacagttcaa agtaaaatcc tgacaactgg agagaactat aaaacagtta
     2461 atgatattgt gtcagcactt cttaatgctg aagatgaaaa aagagaagag gagaaggaaa
     2521 aacaagctga agaaatggca tcagatgatt tgtcattaat tcggaagaac agaatggctc
     2581 tctttcaaca attgacatgt gtgcttccta tcctggataa tcttttaaag gccaatgtaa
     2641 ttaataaaca ggaacatgat attattaaac aaaaaacaca gataccttta caagcgagag
     2701 aactgattga taccattttg gttaaaggaa atgctgcggc caacatcttc aaaaactgtc
     2761 taaaagaaat tgactctaca ttgtataaga acttatttgt ggataagaat atgaagtata
     2821 ttccaacaga agatgtttca ggtctgtcac tggaagaaca attgaggagg ttgcaagaag
     2881 aacgaacttg taaagtgtgt atggacaaag aagtttctgt tgtatttatt ccttgtggtc
     2941 atctggtagt atgccaggaa tgtgcccctt ctctaagaaa atgccctatt tgcaggggta
     3001 taatcaaggg tactgttcgt acatttctct cttacccaac tttcttgtac aaagttggca
     3061 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata
     3121 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag
     3181 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata
     3241 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc
     3301 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga
     3361 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     3421 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     3481 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     3541 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     3601 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat
     3661 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     3721 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     3781 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     3841 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     3901 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     3961 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     4021 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     4081 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     4141 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     4201 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     4261 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     4321 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     4381 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc
     4441 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     4501 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     4561 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     4621 gcttcagcag agcgcagata ccaa
//
