LOCUS       sequence-164060-        3385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses LuxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-LuxR
FEATURES             Location/Qualifiers
     source          1..3385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(371..376,377..393,394..400)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      1335..1406
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1422..1449
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1482..1499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1561..1660)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1677..1696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1678..1696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1701..1717)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1701..1717)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2148..2939
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2968..3324
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     3362..3379
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3363..3379
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattggta
      121 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga
      181 attcatgaga tctgaattca aaagatctta agtaagtaag agtatacgta tatcggctaa
      241 taacgtatta aggcgcttcg gcgccttttt ttatgggggt attttcatcc caatccacac
      301 gtccaacgca cagcaaacac cacgtcgacc ctatcagctg cgtgctttct atgagtcgtt
      361 gctgcataac ttgacaatta atcatccggc tcgtataatg tgtggaaggg cccaagttca
      421 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgctgg
      481 ggagggtttc taatgatata taacacgcaa aacttgcgac aaacaatagg taaggataaa
      541 gagatgggta tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa
      601 gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat
      661 tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca
      721 attctagata attaccctaa aaaatggagg caatattatg atgacgctaa tttaataaaa
      781 tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg gaatatattt
      841 gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa aacatcaggt
      901 cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg aatgcttagt
      961 tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc gtgtatgaac
     1021 ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat agcaaataat
     1081 aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga
     1141 aaaagctctt gggatatttc aaaaatatta ggctgcagtg agcgtactgt cactttccat
     1201 ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat ttctaaagca
     1261 attttaacag gagcaattga ttgcccatac tttaaaaatt aaggatccaa actcgagtaa
     1321 ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1381 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1441 ctttctgcgt ttataggatc ctaactcgct cctcaggctt cctcgctcac tgactcgctg
     1501 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatcaattcg
     1561 acccagcttt cttgtacaaa gttggcatta taaaaaataa ttgctcatca atttgttgca
     1621 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     1681 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     1741 atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc tagaccagcc
     1801 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
     1861 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
     1921 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     1981 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
     2041 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     2101 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     2161 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     2221 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     2281 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     2341 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     2401 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     2461 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     2521 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     2581 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     2641 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     2701 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     2761 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     2821 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     2881 agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataac
     2941 cctcgagagc agttcaacct gttgatagta cgtactaagc tctcatgttt cacgtactaa
     3001 gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc atggctaacg
     3061 tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa gctctcatgt
     3121 ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta
     3181 taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg ttgtggacaa
     3241 caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga
     3301 cacaggaaca cttaacggct gacatgggaa ttagccatgg gcccgtgcga atcaccctag
     3361 gtgtaaaacg acggccagtc ttaag
//
