LOCUS       sequence-164064-        3280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses TraR(W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs078-TraR(W)
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(371..376,377..393,394..400)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      1230..1301
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1317..1344
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1377..1394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(1456..1555)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1572..1591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1573..1591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1596..1612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1596..1612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2043..2834
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2863..3219
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     3257..3274
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3258..3274
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgggcccc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gatgagcaat gcttttttat aatgccaact ttgtacaaaa aagcaggctc cgaattggta
      121 tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat ctgaagtgga
      181 attcatgaga tctgaattca aaagatctta agtaagtaag agtatacgta tatcggctaa
      241 taacgtatta aggcgcttcg gcgccttttt ttatgggggt attttcatcc caatccacac
      301 gtccaacgca cagcaaacac cacgtcgacc ctatcagctg cgtgctttct atgagtcgtt
      361 gctgcataac ttgacaatta atcatccggc tcgtataatg tgtggaaggg cccaagttca
      421 cttaaaaagg agatcaacaa tgaaagcaat tttcgtactg aaacatctta atcatgctgg
      481 ggagggtttc taatgcagca ctggctggat aagctgactg atctggcggc aattgagggt
      541 gatgaatgca ttctgaaaac tggtctggcg gatattgcgg accacttcgg ttttaccggt
      601 tacgcgtacc tgcacatcca gcaccgtcat attaccgctg tgaccaacta ccaccgtcaa
      661 tggcaatcca cctacttcga taaaaaattc gaggcgctgg acccggtggt taagcgtgcg
      721 cgttctcgca aacacatctt cacttggagc ggtgaacacg aacgtccaac tctgagcaaa
      781 gacgaacgcg cgttctacga ccacgcgtcc gactttggta tccgttctgg catcaccatt
      841 ccgattaaaa cggccaacgg tttcatgtct atgttcacca tggcaagcga taaaccggtt
      901 atcgacctgg accgtgaaat cgacgccgta gctgcggcgg cgaccatcgg tcaaattcac
      961 gcgcgtatct cctttctgcg cacgaccccg acggcggaag acgctgcatg gctggatcct
     1021 aaggaagcta cttacctgcg ttggatcgcc gtaggcaaaa ccatgtggga aatcgcagat
     1081 atcgaaggcg ttaaatacaa ttccgtccgc gtaaaactgc gcgaagctat gaaacgcttc
     1141 gacgtacgtt ctaaagccca cctgacggcc ctggctatcc gtcgcaagct gatctgagga
     1201 tccaaactcg agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1261 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1321 caccttcggg tgggcctttc tgcgtttata ggatcctaac tcgctcctca ggcttcctcg
     1381 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1441 gcggtaatca attcgaccca gctttcttgt acaaagttgg cattataaaa aataattgct
     1501 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1561 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1621 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1681 tcctctagac cagccaggac agaaatgcct cgacttcgct gctgcccaag gttgccgggt
     1741 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1801 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1861 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1921 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1981 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2041 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2101 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2161 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2221 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2281 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2341 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2401 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2461 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2521 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2581 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2641 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2701 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2761 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2821 tagtcggcaa ataaccctcg agagcagttc aacctgttga tagtacgtac taagctctca
     2881 tgtttcacgt actaagctct catgtttaac gtactaagct ctcatgttta acgaactaaa
     2941 ccctcatggc taacgtacta agctctcatg gctaacgtac taagctctca tgtttcacgt
     3001 actaagctct catgtttgaa caataaaatt aatataaatc agcaacttaa atagcctcta
     3061 aggttttaag ttttataaga aaaaaaagaa tatataaggc ttttaaagct tttaaggttt
     3121 aacggttgtg gacaacaagc cagggatgta acgcactgag aagcccttag agcctctcaa
     3181 agcaattttg agtgacacag gaacacttaa cggctgacat gggaattagc catgggcccg
     3241 tgcgaatcac cctaggtgta aaacgacggc cagtcttaag
//
