LOCUS       sequence-164071-        3610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses RpaR and inducibly expresses RpaI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Scott SR, Hasty J
  TITLE     Quorum Sensing Communication Modules for Microbial Consortia.
  JOURNAL   ACS Synth Biol. 2016 Sep 16;5(9):969-77. doi: 
            10.1021/acssynbio.5b00286. Epub 2016 May 19.
  PUBMED    27172092
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.5b00286"; journalName: "ACS Synth Biol"; date: 
            "2016-09-16- 16"; volume: "5"; issue: "9"; pages: "969-77"
COMMENT     Sequence Label: Bsrs103-RpaR-RpaI
FEATURES             Location/Qualifiers
     source          1..3610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(33..127)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(158..952)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(269..288)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     879..898
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1131..1149
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             2024..2035
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      2716..2802
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2967..3555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3047..3066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttaagggat tttggtcatg actagtgctt ggattctcac caataaaaaa cgcccggcgg
       61 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
      121 aggagtccaa gcgagctctc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
      181 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
      241 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
      301 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
      361 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
      421 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
      481 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
      541 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
      601 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
      661 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
      721 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat
      781 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
      841 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
      901 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
      961 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     1021 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     1081 gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     1141 tcacgaggcc ctttcgtctt cacccctaga cctagccgtc ttcacctcga gtcacaaacg
     1201 gatcaatccg agcgtcaatg ccttggcaac cgccgcggtg cggttggcgg cgtcgagctt
     1261 gcgggcggct tcgatcagat ggaatttcac cgtgcgttcg gtgatgcaga ggattaccga
     1321 gatttcccag gcggtcttgc cctgcgctgc ccattgcagg atctcgcact cgcgcggcgt
     1381 gagccggttg cgccggatcg gctttggccg gctgagtgcg cgcaggcgac tatgcgcgta
     1441 gatgctgacc agctgcatcg cgccgcgcgc ggccgggctg aggtccggat ccttgccggc
     1501 catgctgatc gcggcgctac cgtcgtcgta gtgcagcgga atgcagtaac cctcgaccag
     1561 tccgaattcc gccgcccggg tcatgacccg gtgggcggcc ggatcacggt cgcggtcgta
     1621 gggtgcatcg gaccatacga aaggatgaac cgtggtagcg ccgtggcgcg gcaccggatc
     1681 gaccgcgctg aagttttcgc tgacatacag atcgaaccag tctcgcggcc agccattggc
     1741 cagcgtcagc tccggtagtc cggcattgcg cgacggcagg ccggccatga tgtaggcggt
     1801 aaatccgcag ctcgcgatca gcgattcgaa ccggctgatc agcgccggcg cctcgagccg
     1861 ttcgacggaa tcgacgaatt ccagcgcacg ccgtccccaa agctgatctt cgccgacgat
     1921 catactattg tatcgctggg aatacaatta cttaacataa gcacctgtag gatcgtacag
     1981 gtttacgcaa gaaaatggtt tgttatagtc gaatgaattc attaaagagg agaaaggtac
     2041 catgcaggtt catgtcatcc gtcgagagaa ccgcgcgctc tatgccggtc tgctcgaaaa
     2101 gtacttccgc atccgtcacc agatctacgt cgtcgagcgc ggctggaagg agctcgatcg
     2161 gccggatggc cgcgagatcg atcagttcga caccgaagac gccgtgtatc tgctcggcgt
     2221 cgacaatgac gacatcgtcg ccggcatgcg gatggtgccg accacgtcac cgacgctcct
     2281 cagcgacgtc ttcccgcagc ttgcgctggc aggcccggtg cggcggccgg atgcctacga
     2341 gctgtcgcgg atcttcgtgg taccgcgcaa gcgcggcgag catggcggcc cgcgcgccga
     2401 agccgtgatc caggcggccg cgatggagta cggcctgtcg atcggtctgt cggccttcac
     2461 catcgtgctg gagacctggt ggctgccgcg actggtggac cagggctgga aggcaaagcc
     2521 gctcggcctg cctcaggaca tcaacggatt ctcgaccacc gcagtgatcg tcgacgtcga
     2581 cgacgacgcc tgggtcggca tctgcaatcg ccgctcggtg cccggaccca cgctggaatg
     2641 gcgcgggctc gaagccatcc gccgtcattc gcttccggaa ttccaggtga tttcatgaac
     2701 gcgtgctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2761 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagacctag
     2821 ggcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2881 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2941 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3001 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3061 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3121 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3181 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3241 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3301 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3361 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3421 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3481 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3541 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3601 gaaaactcac
//
