LOCUS       sequence-164073-        3768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BMI1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BMI1_WT
FEATURES             Location/Qualifiers
     source          1..3768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1284..1303)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1285..1303)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1308..1324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1308..1324)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1755..2546
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2639..3227
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3128..3147
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3381..3398
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3557..3584)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3676..3762)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcatcgaaca acgagaatca agatcactga gctaaatccc cacctgatgt
      241 gtgtgctttg tggagggtac ttcattgatg ccacaaccat aatagaatgt ctacattcct
      301 tctgtaaaac gtgtattgtt cgttacctgg agaccagcaa gtattgtcct atttgtgatg
      361 tccaagttca caagaccaga ccactactga atataaggtc agataaaact ctccaagata
      421 ttgtatacaa attagttcca gggcttttca aaaatgaaat gaagagaaga agggattttt
      481 atgcagctca tccttctgct gatgctgcca atggctctaa tgaagataga ggagaggttg
      541 cagatgaaga taagagaatt ataactgatg atgagataat aagcttatcc attgaattct
      601 ttgaccagaa cagattggat cggaaagtaa acaaagacaa agagaaatct aaggaggagg
      661 tgaatgataa aagatactta cgatgcccag cagcaatgac tgtgatgcac ttaagaaagt
      721 ttctcagaag taaaatggac atacctaata ctttccagat tgatgtcatg tatgaggagg
      781 aacctttaaa ggattattat acactaatgg atattgccta catttatacc tggagaagga
      841 atggtccact tccattgaaa tacagagttc gacctacttg taaaagaatg aagatcagtc
      901 accagagaga tggactgaca aatgctggag aactggaaag tgactctggg agtgacaagg
      961 ccaacagccc agcaggaggt attccctcca cctcttcttg tttgcctagc cccagtactc
     1021 cagtgcagtc tcctcatcca cagtttcctc acatttccag tactatgaat ggaaccagca
     1081 acagccccag cggtaaccac caatcttctt ttgccaatag acctcgaaaa tcatcagtaa
     1141 atgggtcatc agcaacttct tctggttgcc taactttctt gtacaaagtt ggcattataa
     1201 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1261 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1321 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1381 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1441 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     1501 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     1561 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     1621 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     1681 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     1741 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     1801 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     1861 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     1921 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     1981 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2041 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2101 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2161 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2221 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2281 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2341 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2401 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     2461 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     2521 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     2581 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2641 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2701 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2761 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2821 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2881 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2941 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3001 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3061 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3121 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3181 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3241 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3301 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3361 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3421 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3481 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     3541 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     3601 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     3661 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     3721 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
