LOCUS       sequence-164074-        4296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing BMPR1B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BMPR1B_WT
FEATURES             Location/Qualifiers
     source          1..4296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1696..1795)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1812..1831)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1813..1831)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1836..1852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1836..1852)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2283..3074
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3167..3755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3656..3675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3909..3926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4085..4112)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4204..4290)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gcttttgcga agtgcaggaa aattaaatgt gggcaccaag aaagaggatg
      241 gtgagagtac agcccccacc ccccgtccaa aggtcttgcg ttgtaaatgc caccaccatt
      301 gtccagaaga ctcagtcaac aatatttgca gcacagacgg atattgtttc acgatgatag
      361 aagaggatga ctctgggttg cctgtggtca cttctggttg cctaggacta gaaggctcag
      421 attttcagtg tcgggacact cccattcctc atcaaagaag atcaattgaa tgctgcacag
      481 aaaggaacga atgtaataaa gacctacacc ctacactgcc tccattgaaa aacagagatt
      541 ttgttgatgg acctatacac cacagggctt tacttatatc tgtgactgtc tgtagtttgc
      601 tcttggtcct tatcatatta ttttgttact tccggtataa aagacaagaa accagacctc
      661 gatacagcat tgggttagaa caggatgaaa cttacattcc tcctggagaa tccctgagag
      721 acttaattga gcagtctcag agctcaggaa gtggatcagg cctccctctg ctggtccaaa
      781 ggactatagc taagcagatt cagatggtga aacagattgg aaaaggtcgc tatggggaag
      841 tttggatggg aaagtggcgt ggcgaaaagg tagctgtgaa agtgttcttc accacagagg
      901 aagccagctg gttcagagag acagaaatat atcagacagt gttgatgagg catgaaaaca
      961 ttttgggttt cattgctgca gatatcaaag ggacagggtc ctggacccag ttgtacctaa
     1021 tcacagacta tcatgaaaat ggttcccttt atgattatct gaagtccacc accctagacg
     1081 ctaaatcaat gctgaagtta gcctactctt ctgtcagtgg cttatgtcat ttacacacag
     1141 aaatctttag tactcaaggc aaaccagcaa ttgcccatcg agatctgaaa agtaaaaaca
     1201 ttctggtgaa gaaaaatgga acttgctgta ttgctgacct gggcctggct gttaaattta
     1261 ttagtgatac aaatgaagtt gacataccac ctaacactcg agttggcacc aaacgctata
     1321 tgcctccaga agtgttggac gagagcttga acagaaatca cttccagtct tacatcatgg
     1381 ctgacatgta tagttttggc ctcatccttt gggaggttgc taggagatgt gtatcaggag
     1441 gtatagtgga agaataccag cttccttatc atgacctagt gcccagtgac ccctcttatg
     1501 aggacatgag ggagattgtg tgcatcaaga agttacgccc ctcattccca aaccggtgga
     1561 gcagtgatga gtgtctaagg cagatgggaa aactcatgac agaatgctgg gctcacaatc
     1621 ctgcatcaag gctgacagcc ctgcgggtta agaaaacact tgccaaaatg tcagagtccc
     1681 aggacattaa actctgccca actttcttgt acaaagttgg cattataaga aagcattgct
     1741 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1801 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1861 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1921 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1981 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     2041 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     2101 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     2161 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     2221 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2281 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2341 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2401 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2461 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2521 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2581 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2641 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2701 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2761 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2821 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2881 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2941 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     3001 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     3061 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     3121 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3781 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3841 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3901 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3961 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4021 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     4081 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     4141 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     4201 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     4261 gtcttccgac tgagcctttc gttttatttg atgcct
//
