LOCUS       sequence-164077-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 
            scaffold domains + salicylate inducible phosphatase (Psal-CusSmut).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH028
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(50..96)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     249..266
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(322..339)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(355..384)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 aaaatattta atttctagac tgcagttgaa gttcaccgac aaacaacaga taaaacgaaa
       61 ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
      121 catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg
      181 ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt ctgttttatc agaccgcttc
      241 tgcgttctga tttaatctgt atcaggctga aaatcttctc tcatccgcca aaacagccaa
      301 gctggagacc gtttaaactc aatgatgatg atgatgatgg tcgacggcgc tattcagatc
      361 ctcttctgag atgagttttt gttcgggccc aagcttctca ttactcgagt taggatccag
      421 cgggtaatgt gataacaaac cttgtccccc gcgcatccga cgttaccgca accgtgcctt
      481 tatgcgcgac aacaatcgat ttcactatcg ccagcgcaat accgctacct tcaccttttc
      541 gctggcggga ggggtcaacg cgatagaaac ggtcaaacaa tcgcggtaag tgctcgggcg
      601 caatgggcgt accggggttt tcgacgataa cttgcaccag gtgatcgacc gtctgacagc
      661 gcactacaat tgtctctccg gttggcgtat aacgcagggc gttcgaaagc aggttgctta
      721 gcgcccgacg cagcatcagc ggatcgcccg cgacctgaca cttgtcgcca acaaaccgca
      781 actccacgcc gcgatcttcc gctaacgcct cgaaaaaatc gaacactttg ccgacttcat
      841 ccgccaggtt gagcattttc ttttcgggga ttagctggtt gttatcggcc tgagcgagaa
      901 acagcatatc gctgaccatt ttcgccattc
//
