LOCUS       sequence-164082-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Hagting A, Den Elzen N, Vodermaier HC, Waizenegger IC, Peters JM, 
            Pines J
  TITLE     Human securin proteolysis is controlled by the spindle checkpoint 
            and reveals when the APC/C switches from activation by Cdc20 to 
            Cdh1.
  JOURNAL   J Cell Biol. 2002 Jun 24;157(7):1125-37. Epub 2002 Jun 17.
  PUBMED    12070128
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2002-06-24"; volume: "157(7)"; pages: "1125-37. Epub 
            2002 Jun 17"
COMMENT     Sequence Label: pEYFP-Pds1-Delta-destruction box(383)
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(48..50,51..53,54..764)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(93..114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(354..373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     701..722
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag cgctaccggt cgccaccatg gtgagcaagg
       61 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      121 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      181 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
      241 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
      301 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      361 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      421 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      481 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
      541 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      601 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
      661 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      721 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
      781 gagctcaagc ttcgaattct gcagtcgacg gtaccatggc tactctgatc tatgttgata
      841 aggaa
//
