LOCUS       sequence-164090-         544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with hygromycin 
            plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC32
FEATURES             Location/Qualifiers
     source          1..544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(350..396)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(385..405)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ggaatcatcc accttagcca tctcgttgga gaagatctct tgcaggtagc agattctgtt
       61 ctttcttcta gtgtatcttc ttctagcagt tctcttcagt ctagtagcct cagcagtctc
      121 tccggaatcg aacagcagag ctccgatcag gttcttcttg atggagtgtc tatcagtgtt
      181 tcccagcacc ttgaacttct tggatggcac cttgtactca tcagtgatca cagcccatcc
      241 cacggagtta gttccgatat ccagtccgat ggagtacttc ttatccatgt cgacctggtg
      301 gcgcgcctcg agggggggcc cggtacccgg ggatcctcta gagtcgaggt cctctccaaa
      361 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
      421 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
      481 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
      541 gagg
//
