LOCUS       sequence-164092-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2x35S promoting Glycing Max codon optimized Cas9 with hygromycin 
            plant selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Michno JM, Wang X, Liu J, Curtin SJ, Kono TJ, Stupar RM
  TITLE     CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a 
            new web-tool and a modified Cas9 enzyme.
  JOURNAL   GM Crops Food. 2015;6(4):243-52. doi: 10.1080/21645698.2015.1106063.
  PUBMED    26479970
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "GM Crops 
            Food."; date: "2015"; volume: "6(4):243-52. doi"; pages: " 
            10.1080/21645698.2015.1106063"
COMMENT     Sequence Label: Cas9 MDC32
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(49..69)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             155..163
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(216..227)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ggcggccgct ctagaactag ttaattaatc tggtctagat catcctccca cctttctctt
       61 cttctttggt tgcagatctc ctcccagttg ggacagatcg attctagtct cgtacagtcc
      121 agtgatggat tggtggatca gagtagcatc cagcacctcc ttagtggaag tgtatctctt
      181 tctatcgata gtagtatcga agtacttgaa agcagctgga gctcccaggt tagtcagagt
      241 gaacaggtgg atgatgttct cagcttgctc tctgattggc ttatctctgt gcttgttgta
      301 agcggacagc accttatcca ggttagcatc agccaggatc actctcttgg agaactcgga
      361 gatttgctcg atgatctcat ccaggtagtg cttgtgttgc tccacgaaca gttgcttttg
      421 ctcgttatcc tctggggatc ccttcagctt ctcgtagtgg gaagccaggt acaggaagtt
      481 cacgtacttg gatggcagag ccagctcgtt tcccttttgc agctctccag cggaagccag
      541 cattctcttt cttccgttct ccagctcgaa cagggagtac tttggcagct tgatgatcag
      601 atccttcttc acctccttgt atcccttagc ctccaggaaa tcgattgggt tcttctcgaa
      661 ggaggatctc tccatgatag tgattcccag cagctccttc acggacttca gcttcttgga
      721 ctttcccttc tccaccttag ccaccaccag cacggagtaa gccacagttg gggaatcgaa
      781 tcctccgtac ttctttggat cccaatcctt ctttctagcg atcagcttat cggagtttct
      841 ctttggcagg atggactcct tggagaatcc tccagtttgc acctcagtct tcttcacgat
      901 gttcacttgt ggcatggaca g
//
