LOCUS       sequence-164107-       13581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged BuGZ in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 13581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUT BuGZ FL
FEATURES             Location/Qualifiers
     source          1..13581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3400..3801
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3815..4510
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    5922..6494
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6089..6106)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6316..6335
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6497..7099
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6996..7016
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           7106..7227
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7255..7269
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7297..7423
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(7518..7536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7556..8144
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7609..7629)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8027..8038)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8351..8584
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8685..8702)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8691..8915
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8961..9389
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9048..9067)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9258..9279
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9398..9418)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9403..9732
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9583..9718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9645..9664
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9781..10806
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9781..10806
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10936..11057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11106..11122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11106..11122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11119..11141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11130..11146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11154..11160,11161..11178,11179..11184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11199..11220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11337..11354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11508..12096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11588..11607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12267..13058,13059..13127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12890..12909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13128..13232)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13280..13414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccgcgtgt ggcatctgaa gcaccaccag cgagcgagag ctagagagaa
     2761 ggaaagccac cgacttcacc gcctccgagc tgctccgggt cgcgggtctg cagcgtctcc
     2821 ggccctccgc gcctacagct caagccacat ccgaaggggg agggagccgg gagctgcgcg
     2881 cggggccgcc ggggggaggg gtggcaccgc ccacgccggg cggccacgaa gggcggggca
     2941 gcgggcgcgc gcccggcggg gggaggggcc gcgcgccgcg cccgctggga attggggccc
     3001 tagggggagg gcggaggcgc cgacgaccgc ggcacttacc gttcgcggcg tggcgcccgg
     3061 tggtccccaa ggggagggaa gggggaggcg gggcgaggac agtgaccgga gtctcctcag
     3121 cggtggcttt tctgcttggc agcctcagcg gctggcgcca aaaccggact ccgcccactt
     3181 cctcgcccct gcggtgcgag ggtgtggaat cctccagacg ctgggggagg gggagttggg
     3241 agcttaaaaa ctagtacccc tttgggacca ctttcagcag cgaactctcc tgtacaccag
     3301 gggtcagttc cacagacgcg ggccaggggt gggtcattgc ggcgtgaaca ataatttgac
     3361 tagaagttga ttcgggtgtt tccggaattc ctagctgcag taacgccatt ttgcaaggca
     3421 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     3481 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     3541 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     3601 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     3661 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     3721 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     3781 gtcctccgac agactgagtc gcccgctgga gaccatggag agcgacgaga gcggcctgcc
     3841 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     3901 gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc aacaagatga agagcaccaa
     3961 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     4021 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     4081 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     4141 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtgatgggca ccggcttccc
     4201 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     4261 gcaccccatg ggcgataacg atctggatgg cagcttcacc cgcaccttca gcctgcgcga
     4321 cggcggctac tacagctccg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     4381 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg aggatcacag
     4441 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccgg atgcagatgc
     4501 cggtgaagaa tacatgggtc gcaagaagaa gaagcagctg aagccgtggt gctggtattg
     4561 taatagagat tttgatgatg agaagatcct tattcagcac caaaaagcaa agcattttaa
     4621 atgccatata tgtcacaaga aattgtatac aggacctggc ttagctattc attgcatgca
     4681 ggtacataaa gaaacaatag atgccgtacc aaatgcaata cctggaagaa cagacataga
     4741 gttggaaata tatggtatgg aaggtattcc agaaaaagac atggatgaaa gacgacgact
     4801 tcttgaacag aaaacacaag aaagtcaaaa aaagaagcaa caagatgatt ctgatgaata
     4861 tgatgatgac gactctgcag cctcaacttc atttcagcca cagcctgttc aacctcagca
     4921 aggttatatt cctccaatgg cacagccagg actgccacca gtaccaggag caccaggaat
     4981 gcctccaggc atacctccat taatgccagg tgttcctcct ctgatgccag gaatgccacc
     5041 agttatgcca ggcatgccac ctggattgca tcatcagaga aaatacaccc agtcattttg
     5101 cggtgaaaac ataatgatgc caatgggtgg aatgatgcca cctggaccag gaataccacc
     5161 tctgatgcct ggaatgccac caggtatgcc cccacctgtt ccacgtcctg gaattcctcc
     5221 aatgactcaa gcacaggctg tttcagcgcc aggtattctt aatagaccac ctgcaccaac
     5281 agcaactgta cctgccccac agcctccagt tactaagcct cttttcccca gtgctggaca
     5341 ggctcaggca gctgtccaag gacctgttgg tacagatttc aaacccttaa atagtacccc
     5401 tgcaacaact acagaacccc caaagcctac attccctgct tatacacagt ctacagcttc
     5461 aacaactagt acaacaaata gtactgcagc taaaccagcg gcttcaataa caagtaagcc
     5521 tgctacactt acaacaacta gtgcaaccag taagttgatc catccagatg aggatatatc
     5581 cctggaagag agaagggcac agttacctaa gtatcaacgt aatcttcctc ggccaggaca
     5641 ggcccccatc ggtaatccac cagttggacc aattggaggt atgatgccac cacggccagg
     5701 catcccacag caacaaggaa tgagaccccc aatgccacct catggtcagt atggtggtca
     5761 tcatcaaggc atgccaggat accttcctgg tgctatgccc ccgtatgggc agggaccgcc
     5821 aatggtgccc ccttaccagg gtgggcctcc tcgacctccg atgggaatga gacctcctgt
     5881 aatgtcgcaa ggtggccgtt actgagcggc cgcaaattcc gcccctctcc ctcccccccc
     5941 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     6001 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     6061 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     6121 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     6181 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     6241 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     6301 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     6361 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     6421 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     6481 aaaacacgat aataccatgg ccaccgagta caagcccacg gtgcgcctcg ccacccgcga
     6541 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     6601 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     6661 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     6721 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg
     6781 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     6841 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     6901 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     6961 tggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7021 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     7081 ccgcaagccc ggtgcctgag tttgtttgaa tgaggcttca gtactttaca gaatcgttgc
     7141 ctgcacatct tggaaacact tgctgggatt acttcttcag gttaacccaa cagaaggctc
     7201 gagaaggtat attgctgttg acagtgagcg atctcgcttg ggcgagagta agtagtgaag
     7261 ccacagatgt acttactctc gcccaagcga gagtgcctac tgcctcggaa ttcaaggggc
     7321 tactttagga gcaattatct tgtttactaa aactgaatac cttgctatct ctttgataca
     7381 tttttacaaa gctgaattaa aatggtataa attaaatcac ttttttcaat tggaagacta
     7441 atgcggccgg ccattactcc gtctcgtgtc ttgttgcata tgtctgctgg tttgtttgat
     7501 gttgtttgcg ggcgggccct atagtgagtc gtattaccta ggacgcgtct ggaacaatca
     7561 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7621 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     7681 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     7741 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     7801 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     7861 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     7921 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
     7981 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8041 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8101 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc
     8161 agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     8221 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     8281 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8341 aagaggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     8401 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8461 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8521 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8581 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8641 tcagagagtg agaggccttg acattgttta aacccgctga tcagcctcga ctgtgccttc
     8701 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     8761 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     8821 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     8881 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     8941 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9001 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9061 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     9121 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9181 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9241 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9301 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     9361 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     9421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9481 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9541 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9661 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9721 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     9781 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     9841 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     9901 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     9961 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
    10021 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
    10081 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
    10141 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
    10201 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
    10261 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
    10321 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
    10381 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
    10441 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
    10501 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
    10561 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
    10621 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
    10681 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
    10741 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
    10801 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
    10861 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
    10921 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    11041 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
    11101 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    11161 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    11221 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    11281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    11341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11401 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11461 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    11521 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    11581 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    11641 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    11701 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    11761 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    11821 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    11881 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    11941 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12001 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12061 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12121 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    12181 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    12241 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    12301 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    12361 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    12421 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    12481 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    12541 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    12601 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    12661 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    12721 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    12781 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    12841 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    12901 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    12961 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    13021 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    13081 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    13141 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    13201 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    13261 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa
    13321 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    13381 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    13441 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    13501 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    13561 atgtactaca aacttagtag t
//
