LOCUS       sequence-164110-       13515 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged mutant BuGZ in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 13515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUT BuGZ delta ZF1
FEATURES             Location/Qualifiers
     source          1..13515
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3400..3801
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3815..4510
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    5856..6428
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6023..6040)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6250..6269
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6431..7033
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6930..6950
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           7040..7161
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7189..7203
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7231..7357
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(7452..7470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7490..8078
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7543..7563)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7961..7972)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8285..8518
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8619..8636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8625..8849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8895..9323
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8982..9001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9192..9213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9332..9352)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9337..9666
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9517..9652
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9579..9598
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9715..10740
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9715..10740
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10870..10991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11040..11056)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11040..11056)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11053..11075)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11064..11080
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11088..11094,11095..11112,11113..11118))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11133..11154
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11271..11288)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11442..12030)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11522..11541)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12201..12992,12993..13061))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12824..12843
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13062..13166)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13214..13348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccgcgtgt ggcatctgaa gcaccaccag cgagcgagag ctagagagaa
     2761 ggaaagccac cgacttcacc gcctccgagc tgctccgggt cgcgggtctg cagcgtctcc
     2821 ggccctccgc gcctacagct caagccacat ccgaaggggg agggagccgg gagctgcgcg
     2881 cggggccgcc ggggggaggg gtggcaccgc ccacgccggg cggccacgaa gggcggggca
     2941 gcgggcgcgc gcccggcggg gggaggggcc gcgcgccgcg cccgctggga attggggccc
     3001 tagggggagg gcggaggcgc cgacgaccgc ggcacttacc gttcgcggcg tggcgcccgg
     3061 tggtccccaa ggggagggaa gggggaggcg gggcgaggac agtgaccgga gtctcctcag
     3121 cggtggcttt tctgcttggc agcctcagcg gctggcgcca aaaccggact ccgcccactt
     3181 cctcgcccct gcggtgcgag ggtgtggaat cctccagacg ctgggggagg gggagttggg
     3241 agcttaaaaa ctagtacccc tttgggacca ctttcagcag cgaactctcc tgtacaccag
     3301 gggtcagttc cacagacgcg ggccaggggt gggtcattgc ggcgtgaaca ataatttgac
     3361 tagaagttga ttcgggtgtt tccggaattc ctagctgcag taacgccatt ttgcaaggca
     3421 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     3481 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     3541 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     3601 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     3661 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     3721 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     3781 gtcctccgac agactgagtc gcccgctgga gaccatggag agcgacgaga gcggcctgcc
     3841 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     3901 gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc aacaagatga agagcaccaa
     3961 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     4021 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     4081 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     4141 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtgatgggca ccggcttccc
     4201 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     4261 gcaccccatg ggcgataacg atctggatgg cagcttcacc cgcaccttca gcctgcgcga
     4321 cggcggctac tacagctccg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     4381 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg aggatcacag
     4441 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccgg atgcagatgc
     4501 cggtgaagaa tacatgggtc gcaagaagaa gaagcagctg aagccgtggt ttaaatgcca
     4561 tatatgtcac aagaaattgt atacaggacc tggcttagct attcattgca tgcaggtaca
     4621 taaagaaaca atagatgccg taccaaatgc aatacctgga agaacagaca tagagttgga
     4681 aatatatggt atggaaggta ttccagaaaa agacatggat gaaagacgac gacttcttga
     4741 acagaaaaca caagaaagtc aaaaaaagaa gcaacaagat gattctgatg aatatgatga
     4801 tgacgactct gcagcctcaa cttcatttca gccacagcct gttcaacctc agcaaggtta
     4861 tattcctcca atggcacagc caggactgcc accagtacca ggagcaccag gaatgcctcc
     4921 aggcatacct ccattaatgc caggtgttcc tcctctgatg ccaggaatgc caccagttat
     4981 gccaggcatg ccacctggat tgcatcatca gagaaaatac acccagtcat tttgcggtga
     5041 aaacataatg atgccaatgg gtggaatgat gccacctgga ccaggaatac cacctctgat
     5101 gcctggaatg ccaccaggta tgcccccacc tgttccacgt cctggaattc ctccaatgac
     5161 tcaagcacag gctgtttcag cgccaggtat tcttaataga ccacctgcac caacagcaac
     5221 tgtacctgcc ccacagcctc cagttactaa gcctcttttc cccagtgctg gacaggctca
     5281 ggcagctgtc caaggacctg ttggtacaga tttcaaaccc ttaaatagta cccctgcaac
     5341 aactacagaa cccccaaagc ctacattccc tgcttataca cagtctacag cttcaacaac
     5401 tagtacaaca aatagtactg cagctaaacc agcggcttca ataacaagta agcctgctac
     5461 acttacaaca actagtgcaa ccagtaagtt gatccatcca gatgaggata tatccctgga
     5521 agagagaagg gcacagttac ctaagtatca acgtaatctt cctcggccag gacaggcccc
     5581 catcggtaat ccaccagttg gaccaattgg aggtatgatg ccaccacggc caggcatccc
     5641 acagcaacaa ggaatgagac ccccaatgcc acctcatggt cagtatggtg gtcatcatca
     5701 aggcatgcca ggataccttc ctggtgctat gcccccgtat gggcagggac cgccaatggt
     5761 gcccccttac cagggtgggc ctcctcgacc tccgatggga atgagacctc ctgtaatgtc
     5821 gcaaggtggc cgttactgag cggccgcaaa ttccgcccct ctccctcccc cccccctaac
     5881 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     5941 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     6001 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     6061 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     6121 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     6181 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     6241 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     6301 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     6361 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     6421 cgataatacc atggccaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6481 cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6541 cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6601 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6661 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggct cgcgcatggc
     6721 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6781 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6841 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgctggggt
     6901 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6961 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     7021 gcccggtgcc tgagtttgtt tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac
     7081 atcttggaaa cacttgctgg gattacttct tcaggttaac ccaacagaag gctcgagaag
     7141 gtatattgct gttgacagtg agcgatctcg cttgggcgag agtaagtagt gaagccacag
     7201 atgtacttac tctcgcccaa gcgagagtgc ctactgcctc ggaattcaag gggctacttt
     7261 aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga tacattttta
     7321 caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattggaag actaatgcgg
     7381 ccggccatta ctccgtctcg tgtcttgttg catatgtctg ctggtttgtt tgatgttgtt
     7441 tgcgggcggg ccctatagtg agtcgtatta cctaggacgc gtctggaaca atcaacctct
     7501 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     7561 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     7621 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     7681 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     7741 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     7801 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     7861 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     7921 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7981 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8041 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga
     8101 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     8161 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     8221 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg
     8281 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8341 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8461 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8521 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8581 agtgagaggc cttgacattg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     8641 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     8701 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     8761 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     8821 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     8881 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     8941 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     9001 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     9061 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     9121 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     9181 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     9241 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     9301 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     9361 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9421 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9481 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9541 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9601 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9661 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgatgaaa
     9721 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     9781 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     9841 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     9901 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     9961 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
    10021 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
    10081 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
    10141 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
    10201 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
    10261 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
    10321 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
    10381 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
    10441 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
    10501 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
    10561 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
    10621 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
    10681 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
    10741 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    10801 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    10861 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    10921 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    10981 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
    11041 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
    11101 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
    11161 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    11221 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    11281 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    11341 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    11401 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    11461 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    11521 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    11581 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    11641 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    11701 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    11761 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    11821 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    11881 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    11941 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    12001 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    12061 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    12121 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    12181 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    12241 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    12301 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    12361 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    12421 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    12481 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    12541 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    12601 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    12661 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    12721 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    12781 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    12841 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    12901 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    12961 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    13021 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    13081 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    13141 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    13201 ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    13261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    13321 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    13381 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    13441 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    13501 tacaaactta gtagt
//
