LOCUS       sequence-164111-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized Cas9 protein and puromycin 
            resistance from EFS promoter and an intergenic BCR/ABL-targeting 
            sgRNA element from U6 promoter. Lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES,
            Sabatini DM
  TITLE     Identification and characterization of essential genes in the human 
            genome.
  JOURNAL   Science. 2015 Oct 15. pii: aac7041.
  PUBMED    26472758
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2015 Oct 15. pii: aac7041."
COMMENT     Sequence Label: lentiCRISPR - Amplicon, BCR/ABL sgRNA 1
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(51..98)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tcgccggcct gcttcagcag gctgaagttg gtggcgccgc tgccgctagc tttctttttc
       61 ttagcttgac cagctttctt agtagcagca ggacgcttgt cgcctcccag ctgagacagg
      121 tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc gtccagcacc
      181 tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt gaaggcggca
      241 ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg ctctctgatg
      301 ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc gtcggccagg
      361 atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta gtgcttgtgc
      421 tgttccacaa acagctgttt ctgctcatta tcctcggggg agcccttcag cttctcatag
      481 tggctggcca ggtacaggaa gttcacatat ttggagggca gggccagttc gtttcccttc
      541 tgcagttcgc cggcagaggc cagcattctc ttccggccgt tttccagctc gaacagggag
      601 tacttaggca gcttgatgat caggtccttt ttcacttctt tgtagccctt ggcttccaga
      661 aagtcgatgg gattcttctc gaagctgctt ctttccatga tggtgatccc cagcagctct
      721 ttcacactct tcagtttctt ggacttgccc ttttccactt tggccaccac cagcacagaa
      781 taggccacgg tggggctgtc gaagccgccg tacttctta
//
