LOCUS       sequence-164112-       13509 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged mutant BuGZ in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13509)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 13509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUT BuGZ delta ZF2
FEATURES             Location/Qualifiers
     source          1..13509
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3400..3801
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3815..4510
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    5850..6422
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6017..6034)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6244..6263
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6425..7027
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6924..6944
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           7034..7155
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7183..7197
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7225..7351
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(7446..7464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7484..8072
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7537..7557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7955..7966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8279..8512
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8613..8630)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8619..8843
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8889..9317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8976..8995)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9186..9207
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9326..9346)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9331..9660
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9511..9646
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9573..9592
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9709..10734
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9709..10734
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10864..10985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11034..11050)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11034..11050)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11047..11069)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11058..11074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11082..11088,11089..11106,11107..11112))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11127..11148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11265..11282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11436..12024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11516..11535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12195..12986,12987..13055))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12818..12837
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13056..13160)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13208..13342
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccgcgtgt ggcatctgaa gcaccaccag cgagcgagag ctagagagaa
     2761 ggaaagccac cgacttcacc gcctccgagc tgctccgggt cgcgggtctg cagcgtctcc
     2821 ggccctccgc gcctacagct caagccacat ccgaaggggg agggagccgg gagctgcgcg
     2881 cggggccgcc ggggggaggg gtggcaccgc ccacgccggg cggccacgaa gggcggggca
     2941 gcgggcgcgc gcccggcggg gggaggggcc gcgcgccgcg cccgctggga attggggccc
     3001 tagggggagg gcggaggcgc cgacgaccgc ggcacttacc gttcgcggcg tggcgcccgg
     3061 tggtccccaa ggggagggaa gggggaggcg gggcgaggac agtgaccgga gtctcctcag
     3121 cggtggcttt tctgcttggc agcctcagcg gctggcgcca aaaccggact ccgcccactt
     3181 cctcgcccct gcggtgcgag ggtgtggaat cctccagacg ctgggggagg gggagttggg
     3241 agcttaaaaa ctagtacccc tttgggacca ctttcagcag cgaactctcc tgtacaccag
     3301 gggtcagttc cacagacgcg ggccaggggt gggtcattgc ggcgtgaaca ataatttgac
     3361 tagaagttga ttcgggtgtt tccggaattc ctagctgcag taacgccatt ttgcaaggca
     3421 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     3481 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     3541 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     3601 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     3661 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     3721 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     3781 gtcctccgac agactgagtc gcccgctgga gaccatggag agcgacgaga gcggcctgcc
     3841 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     3901 gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc aacaagatga agagcaccaa
     3961 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     4021 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     4081 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     4141 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtgatgggca ccggcttccc
     4201 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     4261 gcaccccatg ggcgataacg atctggatgg cagcttcacc cgcaccttca gcctgcgcga
     4321 cggcggctac tacagctccg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     4381 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg aggatcacag
     4441 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccgg atgcagatgc
     4501 cggtgaagaa tacatgggtc gcaagaagaa gaagcagctg aagccgtggt gctggtattg
     4561 taatagagat tttgatgatg agaagatcct tattcagcac caaaaagcaa agcataaaga
     4621 aacaatagat gccgtaccaa atgcaatacc tggaagaaca gacatagagt tggaaatata
     4681 tggtatggaa ggtattccag aaaaagacat ggatgaaaga cgacgacttc ttgaacagaa
     4741 aacacaagaa agtcaaaaaa agaagcaaca agatgattct gatgaatatg atgatgacga
     4801 ctctgcagcc tcaacttcat ttcagccaca gcctgttcaa cctcagcaag gttatattcc
     4861 tccaatggca cagccaggac tgccaccagt accaggagca ccaggaatgc ctccaggcat
     4921 acctccatta atgccaggtg ttcctcctct gatgccagga atgccaccag ttatgccagg
     4981 catgccacct ggattgcatc atcagagaaa atacacccag tcattttgcg gtgaaaacat
     5041 aatgatgcca atgggtggaa tgatgccacc tggaccagga ataccacctc tgatgcctgg
     5101 aatgccacca ggtatgcccc cacctgttcc acgtcctgga attcctccaa tgactcaagc
     5161 acaggctgtt tcagcgccag gtattcttaa tagaccacct gcaccaacag caactgtacc
     5221 tgccccacag cctccagtta ctaagcctct tttccccagt gctggacagg ctcaggcagc
     5281 tgtccaagga cctgttggta cagatttcaa acccttaaat agtacccctg caacaactac
     5341 agaaccccca aagcctacat tccctgctta tacacagtct acagcttcaa caactagtac
     5401 aacaaatagt actgcagcta aaccagcggc ttcaataaca agtaagcctg ctacacttac
     5461 aacaactagt gcaaccagta agttgatcca tccagatgag gatatatccc tggaagagag
     5521 aagggcacag ttacctaagt atcaacgtaa tcttcctcgg ccaggacagg cccccatcgg
     5581 taatccacca gttggaccaa ttggaggtat gatgccacca cggccaggca tcccacagca
     5641 acaaggaatg agacccccaa tgccacctca tggtcagtat ggtggtcatc atcaaggcat
     5701 gccaggatac cttcctggtg ctatgccccc gtatgggcag ggaccgccaa tggtgccccc
     5761 ttaccagggt gggcctcctc gacctccgat gggaatgaga cctcctgtaa tgtcgcaagg
     5821 tggccgttac tgagcggccg caaattccgc ccctctccct cccccccccc taacgttact
     5881 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     5941 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     6001 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     6061 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     6121 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     6181 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     6241 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6301 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6361 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa
     6421 taccatggcc accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
     6481 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     6541 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     6601 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     6661 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggctcgcgca tggccgagtt
     6721 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     6781 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     6841 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgctg gggtgcccgc
     6901 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     6961 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7021 tgcctgagtt tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
     7081 gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat
     7141 tgctgttgac agtgagcgat ctcgcttggg cgagagtaag tagtgaagcc acagatgtac
     7201 ttactctcgc ccaagcgaga gtgcctactg cctcggaatt caaggggcta ctttaggagc
     7261 aattatcttg tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc
     7321 tgaattaaaa tggtataaat taaatcactt ttttcaattg gaagactaat gcggccggcc
     7381 attactccgt ctcgtgtctt gttgcatatg tctgctggtt tgtttgatgt tgtttgcggg
     7441 cgggccctat agtgagtcgt attacctagg acgcgtctgg aacaatcaac ctctggatta
     7501 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7561 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7621 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7681 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7741 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7801 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7861 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     7921 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7981 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8041 gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta
     8101 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     8161 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga
     8221 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg
     8281 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8341 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8401 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8461 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8521 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8581 aggccttgac attgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     8641 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     8701 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     8761 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     8821 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     8881 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8941 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9001 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9061 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9121 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9181 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9241 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9301 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     9361 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     9421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9481 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     9541 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     9601 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9661 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgat gaaaaagcct
     9721 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     9781 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     9841 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     9901 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     9961 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
    10021 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
    10081 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
    10141 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
    10201 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
    10261 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
    10321 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
    10381 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
    10441 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
    10501 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
    10561 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
    10621 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
    10681 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgtg
    10741 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
    10801 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
    10861 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    10921 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    10981 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
    11041 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
    11101 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
    11161 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
    11221 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
    11281 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    11341 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    11401 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    11461 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    11521 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    11581 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    11641 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    11701 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    11761 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    11821 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    11881 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    11941 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    12001 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    12061 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    12121 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    12181 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    12241 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    12301 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    12361 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    12421 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    12481 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    12541 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    12601 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    12661 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    12721 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    12781 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    12841 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    12901 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    12961 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    13021 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    13081 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    13141 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
    13201 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    13261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    13321 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    13381 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    13441 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    13501 cttagtagt
//
