LOCUS       sequence-164117-       13530 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GFP tagged mutant BuGZ in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Toledo CM, Herman JA, Olsen JB, Ding Y, Corrin P, Girard EJ, Olson 
            JM, Emili A, DeLuca JG, Paddison PJ
  TITLE     BuGZ is required for Bub3 stability, Bub1 kinetochore function, and 
            chromosome alignment.
  JOURNAL   Dev Cell. 2014 Feb 10;28(3):282-94. doi: 
            10.1016/j.devcel.2013.12.014. Epub 2014 Jan 23.
  PUBMED    24462187
REFERENCE   2  (bases 1 to 13530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2013.12.014"; journalName: "Dev Cell"; date: 
            "2014-02-10- 10"; volume: "28"; issue: "3"; pages: "282-94"
COMMENT     Sequence Label: GUT BuGZ delta GLEBS
FEATURES             Location/Qualifiers
     source          1..13530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        3400..3801
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3815..4510
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    5871..6443
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6038..6055)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6265..6284
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6446..7048
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6945..6965
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     ncRNA           7055..7176
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7204..7218
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           7246..7372
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(7467..7485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7505..8093
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7558..7578)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7976..7987)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8300..8533
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8634..8651)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8640..8864
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8910..9338
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8997..9016)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9207..9228
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9347..9367)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9352..9681
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9532..9667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9594..9613
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9730..10755
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9730..10755
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    10885..11006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11055..11071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11055..11071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11068..11090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11079..11095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11103..11109,11110..11127,11128..11133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11148..11169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11286..11303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11457..12045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11537..11556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12216..13007,13008..13076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12839..12858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13077..13181)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13229..13363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gaccgcgtgt ggcatctgaa gcaccaccag cgagcgagag ctagagagaa
     2761 ggaaagccac cgacttcacc gcctccgagc tgctccgggt cgcgggtctg cagcgtctcc
     2821 ggccctccgc gcctacagct caagccacat ccgaaggggg agggagccgg gagctgcgcg
     2881 cggggccgcc ggggggaggg gtggcaccgc ccacgccggg cggccacgaa gggcggggca
     2941 gcgggcgcgc gcccggcggg gggaggggcc gcgcgccgcg cccgctggga attggggccc
     3001 tagggggagg gcggaggcgc cgacgaccgc ggcacttacc gttcgcggcg tggcgcccgg
     3061 tggtccccaa ggggagggaa gggggaggcg gggcgaggac agtgaccgga gtctcctcag
     3121 cggtggcttt tctgcttggc agcctcagcg gctggcgcca aaaccggact ccgcccactt
     3181 cctcgcccct gcggtgcgag ggtgtggaat cctccagacg ctgggggagg gggagttggg
     3241 agcttaaaaa ctagtacccc tttgggacca ctttcagcag cgaactctcc tgtacaccag
     3301 gggtcagttc cacagacgcg ggccaggggt gggtcattgc ggcgtgaaca ataatttgac
     3361 tagaagttga ttcgggtgtt tccggaattc ctagctgcag taacgccatt ttgcaaggca
     3421 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     3481 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     3541 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     3601 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     3661 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     3721 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     3781 gtcctccgac agactgagtc gcccgctgga gaccatggag agcgacgaga gcggcctgcc
     3841 cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt tcgagctggt
     3901 gggcggcgga gagggcaccc ccgagcaggg ccgcatgacc aacaagatga agagcaccaa
     3961 aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg gcttctacca
     4021 cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca acaacggcgg
     4081 ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg tgagcttcag
     4141 ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtgatgggca ccggcttccc
     4201 cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg tggagcacct
     4261 gcaccccatg ggcgataacg atctggatgg cagcttcacc cgcaccttca gcctgcgcga
     4321 cggcggctac tacagctccg tggtggacag ccacatgcac ttcaagagcg ccatccaccc
     4381 cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg aggatcacag
     4441 caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccgg atgcagatgc
     4501 cggtgaagaa tacatgggtc gcaagaagaa gaagcagctg aagccgtggt gctggtattg
     4561 taatagagat tttgatgatg agaagatcct tattcagcac caaaaagcaa agcattttaa
     4621 atgccatata tgtcacaaga aattgtatac aggacctggc ttagctattc attgcatgca
     4681 ggtacataaa gaaacaatag atgccgtacc aaatgcaata cctggaagaa cagacataga
     4741 gttggaaata tatggtatgg aaggtattcc agaaaaagac atggatgaaa gacgacgact
     4801 tcttgaacag aaaacacaag aaagtcaaaa aaagaagcaa caagatgatt ctgatgaata
     4861 tgatgatgac gactctgcag cctcaacttc atttcagcca cagcctgttc aacctcagca
     4921 aggttatatt cctccaatgg cacagccagg actgccacca gtaccaggag caccaggaat
     4981 gcctccaggc atacctccat taatgccagg tgttcctcct ctgatgccag gaatgccacc
     5041 agttatgcca ggcatgccac ctggattgca tcatcagaga aaatacaccc agtcattttg
     5101 cggtgaaaac ataatgatgc caatgggtgg aatgatgcca cctggaccag gaataccacc
     5161 tctgatgcct ggaatgccac caggtatgcc cccacctgtt ccacgtcctg gaattcctcc
     5221 aatgactcaa gcacaggctg tttcagcgcc aggtattctt aatagaccac ctgcaccaac
     5281 agcaactgta cctgccccac agcctccagt tactaagcct cttttcccca gtgctggaca
     5341 ggctcaggca gctgtccaag gacctgttgg tacagatttc aaacccttaa atagtacccc
     5401 tgcaacaact acagaacccc caaagcctac attccctgct tatacacagt ctacagcttc
     5461 aacaactagt acaacaaata gtactgcagc taaaccagcg gcttcaataa caagtaagcc
     5521 tgctacactt acaacaacta gtgcaaccag taagttgatc catccagatg aggatatatc
     5581 cccaggacag gcccccatcg gtaatccacc agttggacca attggaggta tgatgccacc
     5641 acggccaggc atcccacagc aacaaggaat gagaccccca atgccacctc atggtcagta
     5701 tggtggtcat catcaaggca tgccaggata ccttcctggt gctatgcccc cgtatgggca
     5761 gggaccgcca atggtgcccc cttaccaggg tgggcctcct cgacctccga tgggaatgag
     5821 acctcctgta atgtcgcaag gtggccgtta ctgagcggcc gcaaattccg cccctctccc
     5881 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     5941 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     6001 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     6061 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     6121 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     6181 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     6241 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     6301 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     6361 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     6421 ttcctttgaa aaacacgata ataccatggc caccgagtac aagcccacgg tgcgcctcgc
     6481 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6541 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6601 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6661 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6721 cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6781 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6841 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6901 cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6961 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7021 gtgcatgacc cgcaagcccg gtgcctgagt ttgtttgaat gaggcttcag tactttacag
     7081 aatcgttgcc tgcacatctt ggaaacactt gctgggatta cttcttcagg ttaacccaac
     7141 agaaggctcg agaaggtata ttgctgttga cagtgagcga tctcgcttgg gcgagagtaa
     7201 gtagtgaagc cacagatgta cttactctcg cccaagcgag agtgcctact gcctcggaat
     7261 tcaaggggct actttaggag caattatctt gtttactaaa actgaatacc ttgctatctc
     7321 tttgatacat ttttacaaag ctgaattaaa atggtataaa ttaaatcact tttttcaatt
     7381 ggaagactaa tgcggccggc cattactccg tctcgtgtct tgttgcatat gtctgctggt
     7441 ttgtttgatg ttgtttgcgg gcgggcccta tagtgagtcg tattacctag gacgcgtctg
     7501 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     7561 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     7621 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     7681 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7741 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7801 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7861 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     7921 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7981 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     8041 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     8101 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     8161 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     8221 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8281 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     8341 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8521 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8581 aaatgaatat cagagagtga gaggccttga cattgtttaa acccgctgat cagcctcgac
     8641 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     8701 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     8761 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     8821 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     8881 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     8941 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     9001 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     9061 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     9121 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     9181 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     9241 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     9301 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     9361 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9421 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     9481 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     9541 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9601 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     9661 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     9721 cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     9781 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     9841 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     9901 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     9961 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
    10021 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
    10081 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
    10141 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
    10201 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
    10261 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
    10321 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
    10381 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
    10441 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
    10501 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
    10561 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
    10621 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
    10681 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
    10741 agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
    10801 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    10861 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    10921 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10981 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    11041 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    11101 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    11161 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    11221 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    11281 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    11341 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    11401 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    11461 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    11521 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    11581 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    11641 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    11701 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11761 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11821 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11881 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11941 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    12001 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    12061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    12121 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    12181 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    12241 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    12301 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    12361 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    12421 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    12481 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    12541 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    12601 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    12661 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    12721 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    12781 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    12841 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    12901 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12961 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    13021 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    13081 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    13141 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    13201 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
    13261 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    13321 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
    13381 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
    13441 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
    13501 ttgttaaata tgtactacaa acttagtagt
//
