LOCUS       sequence-164135-         981 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Staphylococcus aureus (SaCas9) conjugated with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW
  TITLE     Genome editing using CRISPR-Cas9 to create the HPFH genotype in 
            HSPCs: An approach for treating sickle cell disease and 
            beta-thalassemia.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 
            10.1073/pnas.1612075113. Epub 2016 Sep 6.
  PUBMED    27601644
REFERENCE   2  (bases 1 to 981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1612075113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-09-20- 20"; volume: "113"; issue: "38"; pages: "10661-5"
COMMENT     Sequence Label: pX601-mCherry
FEATURES             Location/Qualifiers
     source          1..981
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(116..169)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(170..217)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accattgggc
      121 caggattctc ctcgacgtca ccacatgtta gcagggatcc tctgccctcc tttttctttt
      181 ttgcctggcc ggcctttttc gtggccgccg gccttttgcc ctttttgatg atctgagggt
      241 gcttcttaga tttcacttca tacaggttgc ccagaatgtc tgtgctgtac ttcttaatgc
      301 tctgggtctt ggaggcgatt gtcttaatga tcctgggggg cctcttgtcg ttcatgtttt
      361 ccaggtactc gcggtaggtg atgtcgatca tgttcacttc gatccggttc agcaggtcgt
      421 tgttcacgcc gatcactcta tacagctcgc cgttgatctt gatcagatcg ttgttgtaga
      481 aggaggcgat aaactcggcc tggttgctga tcttcttcag cttcttagct tcctcatagc
      541 acttgctatt cacttcgtag tagttttctt ttttgatcac atccagattc ttcacggtca
      601 cgaacttgta cacgccattg tccaggtaca cgtcgaatct gtagggcttc agggacagct
      661 tcacgacctt gtttctgctg ttggggtagt cgtcggtgat gtccagatgg gcgttcagtt
      721 tgttgccgta atacttaatc ttcttgatca cggggccgtt gtcctttttg gagtacttgg
      781 tcaggtagtt cccggtttcc tcgtagtact tgtacagggg attcttctcg tcgccgtact
      841 gttccataat cagcttcagt ttctggtagg tctgggggtc gtggtggtac atcagcagct
      901 tttcggggct cttgttgatc agctttttca gcttgtcatt gtccttgtcg tacaggccgt
      961 tcagattgtt cacgatcagg g
//
