LOCUS       sequence-164138-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Staphylococcus aureus (SaCas9) conjugated with GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Ye L, Wang J, Tan Y, Beyer AI, Xie F, Muench MO, Kan YW
  TITLE     Genome editing using CRISPR-Cas9 to create the HPFH genotype in 
            HSPCs: An approach for treating sickle cell disease and 
            beta-thalassemia.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Sep 20;113(38):10661-5. doi: 
            10.1073/pnas.1612075113. Epub 2016 Sep 6.
  PUBMED    27601644
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1612075113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-09-20- 20"; volume: "113"; issue: "38"; pages: "10661-5"
COMMENT     Sequence Label: pX601-GFP
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..76)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(77..124)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gctcctcgcc cttgctcacc attgggccag gattctcctc gacgtcacca catgttagca
       61 gggatcctct gccctccttt ttcttttttg cctggccggc ctttttcgtg gccgccggcc
      121 ttttgccctt tttgatgatc tgagggtgct tcttagattt cacttcatac aggttgccca
      181 gaatgtctgt gctgtacttc ttaatgctct gggtcttgga ggcgattgtc ttaatgatcc
      241 tggggggcct cttgtcgttc atgttttcca ggtactcgcg gtaggtgatg tcgatcatgt
      301 tcacttcgat ccggttcagc aggtcgttgt tcacgccgat cactctatac agctcgccgt
      361 tgatcttgat cagatcgttg ttgtagaagg aggcgataaa ctcggcctgg ttgctgatct
      421 tcttcagctt cttagcttcc tcatagcact tgctattcac ttcgtagtag ttttcttttt
      481 tgatcacatc cagattcttc acggtcacga acttgtacac gccattgtcc aggtacacgt
      541 cgaatctgta gggcttcagg gacagcttca cgaccttgtt tctgctgttg gggtagtcgt
      601 cggtgatgtc cagatgggcg ttcagtttgt tgccgtaata cttaatcttc ttgatcacgg
      661 ggccgttgtc ctttttggag tacttggtca ggtagttccc ggtttcctcg tagtacttgt
      721 acaggggatt cttctcgtcg ccgtactgtt ccataatcag cttcagtttc tggtaggtct
      781 gggggtcgtg gtggtacatc agcagctttt cggggctctt gttgatcagc tttttcagct
      841 tgtcattgtc cttgtcgtac aggccgttca gattgttcac gatcagggtg ttgcccttgt
      901 cgtccttccg ggtggagtac agggtgtcgt taatcagctc tctatta
//
