LOCUS       sequence-164141-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression vector encoding murine Tal1 downstream of a 
            doxycycline inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA
  TITLE     An expandable, inducible hemangioblast state regulated by fibroblast
            growth factor.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 
            10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
  PUBMED    25458896
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.10.003"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "1043-57"
COMMENT     Sequence Label: PB-Tal1
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   5..67
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    439..457
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    476..494
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    513..531
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    550..568
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    587..605
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    624..642
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    661..679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    698..716
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     738..762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    842..866
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg cgtaaaattg
       61 acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa
      121 gttttattat atttacactt acatactaat aataaattca acaaacaatt tatttatgtt
      181 tatttattta ttaaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt
      241 taaacattct ctcttttaca aaaataaact tattttgtac tttaaaaaca gtcatgttgt
      301 aatcgataat aagtaattag cttaacctat acataataga aacaaattat acttattagt
      361 cagtcagaaa caactttggc acatatcaat attatgctct cgttaattaa ctcgagggat
      421 ccaagctggc cgcacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
      481 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
      541 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
      601 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc
      661 tccctatcag tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt
      721 accccctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
      781 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccgcgg tggcggccat
      841 cacaagtttg tacaaaaaag caggctccga attcgccctt aggatgacgg agcggccgcc
      901 gagcgaggcg gcacgcagtg acccgcaact agagggacag gacgcggccg aggcccgcat
      961 ggcccccccg cacctagtcc tgctcaacgg cgtcgccaag gagacgagcc gcgcagcccc
     1021 ggctgagccc c
//
