LOCUS       sequence-164142-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dRGA[MCS]
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..820)
                     /codon_start=1
                     /product="GFP variant optimized for excitation by UV light"
                     /label=GFPuv
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(147..167)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     762..790
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctcgcaatg acgcttctca aaactacatt ataaagcaaa aaagcctata tagtcaaact
       61 atataggctt tttttatgat tatagaacgg ttgcttatta gccttatttg tagagctcat
      121 ccatgccatg tgtaatccca gcagcagtta caaactcaag aaggaccatg tggtcacgct
      181 tttcgttggg atctttcgaa agggcagatt gtgtcgacag gtaatggttg tctggtaaaa
      241 ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacggatc
      301 catcttcaat gttgtggcga attttgaagt tagctttgat tccattcttt tgtttgtctg
      361 ccgtgatgta tacattgtgt gagttatagt tgtactcgag tttgtgtccg agaatgtttc
      421 catcttcttt aaaatcaata ccttttaact cgatacgatt aacaagggta tcaccttcaa
      481 acttgacttc agcacgcgtc ttgtagttcc cgtcatcttt gaaagatata gtgcgttcct
      541 gtacataacc ttcgggcatg gcactcttga aaaagtcatg ccgtttcata tgatccggat
      601 aacgggaaaa gcattgaaca ccataagaga aagtagtgac aagtgttggc catggaacag
      661 gtagttttcc agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt
      721 caccctctcc actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa
      781 ttgggacaac tccagtgaaa agttcttctc ctttactcat atgtaaaacc ttaatcaaaa
      841 ttaaatcact taaaaagtgt tataacgcaa cttttttaat atgtctatta taattaataa
      901 cata
//
