LOCUS       sequence-164143-         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dSGK[MCS]
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(365..393)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg cgagatcacc
       61 aaggtagtcg gcaaataagt taagcaaccg ttctataatc ataaaaaaag cctatatagt
      121 ttgactatat aggctttttt gctttataat gtagttttga gaagcgtcat tgcgaggatc
      181 ctctagagga tccgcaacaa ggccaacacc gctacggaat gacatataat ctatacataa
      241 aaatatcatc tatgttatta attataatag acatattaaa aaagttgcgt tataacactt
      301 tttaagtgat ttaattttga ttaaggtttt acatatgagt aaaggagaag aacttttcac
      361 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      421 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      481 tactggaaaa ctacctgttc catggccaac acttgtcact actttctctt atggtgttca
      541 atgcttttcc cgttatccgg atcatatgaa acggcatgac tttttcaaga gtgccatgcc
      601 cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggaact acaagacgcg
      661 tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa aaggtattga
      721 ttttaaagaa gatggaaaca ttctcggaca caaactcgag tacaactata actcacacaa
      781 tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca
      841 caacattgaa gatggatccg ttcaactagc agaccattat caacaaaata ctccaattgg
      901 cga
//
