LOCUS       sequence-164144-         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dSGK[MCS]
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(156..176)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctggggatc ctcgcaatga cgcttctcaa aactacatta taaagcaaaa aagcctatat
       61 agtcaaacta tataggcttt ttttatgatt atagaacggt tgcttattag ccttatttgt
      121 agagctcatc catgccatgt gtaatcccag cagcagttac aaactcaaga aggaccatgt
      181 ggtcacgctt ttcgttggga tctttcgaaa gggcagattg tgtcgacagg taatggttgt
      241 ctggtaaaag gacagggcca tcgccaattg gagtattttg ttgataatgg tctgctagtt
      301 gaacggatcc atcttcaatg ttgtggcgaa ttttgaagtt agctttgatt ccattctttt
      361 gtttgtctgc cgtgatgtat acattgtgtg agttatagtt gtactcgagt ttgtgtccga
      421 gaatgtttcc atcttcttta aaatcaatac cttttaactc gatacgatta acaagggtat
      481 caccttcaaa cttgacttca gcacgcgtct tgtagttccc gtcatctttg aaagatatag
      541 tgcgttcctg tacataacct tcgggcatgg cactcttgaa aaagtcatgc cgtttcatat
      601 gatccggata acgggaaaag cattgaacac cataagagaa agtagtgaca agtgttggcc
      661 atggaacagg ta
//
