LOCUS       sequence-164145-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified from pCFD4, with the vermillion marker and the attB site 
            removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Ge DT, Tipping C, Brodsky MH, Zamore PD
  TITLE     Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome
            Using white Co-conversion.
  JOURNAL   G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557.
  PUBMED    27543296
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 
            10.1534/g3.116.032557."
COMMENT     Sequence Label: pCFD4d
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        196..271
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        272..692
                     /label=dU6-3 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-3 
                     snRNA (Port et al., 2014)"
     misc_RNA        711..786
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gtgtgcaccc aaaagggatt cctatccggc ggtagtccca tcattggcat ggtaggtacc
       61 cgaaagcaac cccttagtta cagacacagc gcgtacgtcc ttcgcatcct tatgattccc
      121 aagtacatat tctgcaagag tacagtatat ataggaaaga tatccgggtg aacttcgggg
      181 tcttcgagaa gacctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
      241 tcaacttgaa aaagtggcac cgagtcggtg cttttttgct cacctgtgat tgctcctact
      301 caaatacaaa aacatcaaat tttctgtcaa taaagcatat ttatttatat ttattttaca
      361 ggaaagaatt ccttttaaag tgtattttaa cctataatga aaaacgatta aaaaaaatac
      421 ataaaataat tcgaaaattt ttgaatagcc caggttgata aaaattcatt tcatacgttt
      481 tataacttat gcccctaagt attttttgac catagtgttt caattctaca ttaattttac
      541 agagtagaat gaaacgccac ctactcagcc aagaggcgaa aaggttagct cgccaagcag
      601 agagggcgcc agtgctcact actttttata attctcaact tctttttcca gactcagttc
      661 gtatatatag acctattttc aatttaacgt cggggtcttc gagaagacct gttttagagc
      721 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
      781 cggtgctttt ttgcctacct ggagcctgag agttgttcaa taaaataaaa atgtttcgtt
      841 tttttgcttt cgccagtatt tattattttt catcaatatg tattcaattt ggtatgtatt
      901 tagtaattgt aatatataga caatggtttt ccgttgacgt acatacatct gacgtgtgtt
      961 tatttagaca taatagttat gttttcacat c
//
