LOCUS       sequence-164146-        3020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the coding region to express the EcoRI restriction enzyme. 
            The RTX tag allows the EcoRI enzyme to be isolated via 
            calcium-precipitation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 2 to 36)
  AUTHORS   Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, 
            Zimmermann SA, Brasino M, Rokicki J, Dowell RD
  TITLE     Engineered calcium-precipitable restriction enzyme.
  JOURNAL   ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m.
  PUBMED    25524101
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Synth Biol"; date: "2014-12-19- 19"; volume: "3"; issue: "12"; 
            pages: "969-71"
COMMENT     Sequence Label: pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
FEATURES             Location/Qualifiers
     source          1..3020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2..36
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             45..56
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 gttgacggct agctcagtcc taggtacagt gctagctact agagaaagag gagaaatact
       61 agatggccgg cgcacgcaac gcgaccaata agctcctgca caaagcgaaa aaaagcaaaa
      121 gcgatgagtt ttatacccag tattgcgaca ttgaaaacga attacaatac taccgcgaac
      181 atttcagcga taaggttgtg tactgcaact gtgatgaccc gcgtgtgagc aattttttta
      241 aatatttcgc ggtgaacttt gacaacctcg gactgaagaa gctgattgca agctgttatg
      301 tagaaaacaa ggagggcttt agctccagcg aagcagcaaa aaatggcttt tactatgaat
      361 accataaaga aaacggcaaa aaactggtct tcgatgatat ttcggtgagt agcttctgtg
      421 gcgacggaga ttttcggagc agtgaaagca ttgatctgct caaaaaaagt gatatcgtgg
      481 tcacgaaccc gcctttttca ctctttcgtg aatacttaga tcaacttatt aaatacgata
      541 aaaagttcct tatcatcgcc aatgtaaaca gcattactta taaagaagtg tttaatctga
      601 tcaaagaaaa caagatctgg ctgggagtac atttaggccg gggtgtgagt ggcttcatcg
      661 ttccagaaca ttacgaactg tacggcaccg aggcccgcat tgattcaaat ggaaaccgta
      721 ttatcagccc gaataactgt ctgtggctga cgaaccttga cgtcttcatt cgccacaaag
      781 acctgccgtt aacacgtaaa tactttggaa acgaaagctc gtacccaaag tatgataact
      841 atgatgcgat taacgttaac aagacgaagg atattccgct ggattataat ggcgttatgg
      901 gggtgccgat caccttcctg cataaattca atcctgaaca atttgaattg attaaattcc
      961 ggaaaggtgt ggatgagaaa gacctatcaa ttaacggaaa gtgcccatat tttcgaatcc
     1021 tgattaaaaa taagcgctta cagaaaaccg gcagtgtgat cgctaaacaa atgacctaca
     1081 aggtttatat gtcaggcacg gtcaatggac actactttga ggtcgaaggc gatggaaaag
     1141 gtaagcccta cgagggggag cagacggtaa agctcactgt caccaagggc ggacctctgc
     1201 catttgcttg ggatatttta tcaccacagt gtcagtacgg aagcatacca ttcaccaagt
     1261 accctgaaga catccctgac tatgtaaagc agtcattccc ggagggctat acatgggaga
     1321 ggatcatgaa ctttgaagat ggtgcagtgt gtactgtcag caatgattcc agcatccaag
     1381 gcaactgttt catctaccat gtcaagttct ctggtttgaa ctttcctccc aatggacctg
     1441 tcatgcagaa gaagacacag ggctgggaac ccaacactga gcgtctcttt gcacgagatg
     1501 gaatgctgct aggaaacaac tttatggctc tgagttagaa ggaggcggtc actatttgtg
     1561 tgaatttaaa actacttaca aggcaaagaa gcctgtgaag atgccagggt atcactatgt
     1621 tgaccgcaaa ctggatgtaa ccaatcacaa caaggattac acttcggttg agcagtgtga
     1681 aatttccatt gcacgcaaac ctgtggtcgc caccggttaa tactagatgg ccggcagcaa
     1741 caaaaaacag agcaatcgcc tgactgagca gcacaaactg agccaaggtg ttattggcat
     1801 ctttggcgat tatgccaaag cccacgacct ggctgtgggg gaggtaagca aattggttaa
     1861 gaaggcactg agcaatgaat acccgcagct gtcgttccgc tatcgtgata gcatcaaaaa
     1921 aacggaaata aatgaagctc tcaaaaagat tgatccagac ctgggtggca ctctttttgt
     1981 atcgaatagc tctattaagc ctgatggcgg gattgtggaa gtgaaggatg attacgggga
     2041 gtggcgcgtc gtgttagttg ccgaagcaaa gcaccagggt aaggatatca tcaacattcg
     2101 taatggcttg cttgtcggca aacgtggtga ccaggacctg atggccgcgg gcaacgccat
     2161 cgagcgtagc cataaaaata tctcagaaat cgcaaatttc atgttatcag aaagccattt
     2221 tccatatgtg ttatttctgg agggctcaaa tttcttgacc gaaaatatca gcattacccg
     2281 tccggatggt cgcgtggtta acctagaata taacagcggc atcctgaatc gtctggatcg
     2341 tctgaccgcg gctaactatg gcatgccaat caactctaac ctgtgcataa acaaattcgt
     2401 gaatcataaa gataaatcta ttatgctcca ggcggcgagc atttataccc agggggatgg
     2461 tcgcgaatgg gattcgaaaa tcatgttcga gattatgttt gatattagca ctacgtcact
     2521 tcgcgtgctg ggtcgcgatc tattcgagca gctgactagc aaaaccggta tggccggcgg
     2581 tgcgggcaac gataccctgt atggtggcgc cgggaatgac acattatacg gaggtgctgg
     2641 caatgatacg ctgtatggcg gagcaggtaa cgacactttg tatgggggcg ccggtaacga
     2701 cacgctgtat gggggggcag gcaacgatac cctttatggc ggtgctggga atgacacact
     2761 gtacggcggg gcgggtaacg ataccctcta taccggcggt gcgggcaacg ataccctgta
     2821 tggtggcgcc gggaatgaca cattatacgg aggtgctggc aatgatacgc tgtatggcgg
     2881 agcaggtaac gacactttgt atgggggcgc cggtaacgac acgctgtatg ggggggcagg
     2941 caacgatacc ctttatggcg gtgctgggaa tgacacactg tacggcgggg cgggtaacga
     3001 taccctctat accggttaat
//
