LOCUS       sequence-164147-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used for the transformation of rickettsia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Burkhardt NY, Baldridge GD, Williamson PC, Billingsley PM, Heu CC, 
            Felsheim RF, Kurtti TJ, Munderloh UG
  TITLE     Development of shuttle vectors for transformation of diverse 
            Rickettsia species.
  JOURNAL   PLoS One. 2011;6(12):e29511. doi: 10.1371/journal.pone.0029511. Epub
            2011 Dec 21.
  PUBMED    22216299
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12):e29511. doi"; pages: " 
            10.1371/journal.pone.0029511. Epub 2011 Dec 21"
COMMENT     Sequence Label: pRAM18dSFA[MCS]
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(161..181)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tgcaagctgg ggatcctcgc aatgacgctt ctcaaaacta cattataaag caaaaaagcc
       61 tatatagtca aactatatag gcttttttta tgattataga acggttgctt attagcctta
      121 tttgtagagc tcatccatgc catgtgtaat cccagcagca gttacaaact caagaaggac
      181 catgtggtca cgcttttcgt tgggatcttt cgaaagggca gattgtgtcg actaatcatc
      241 tatgccctaa tttgctagga agatcacaat aacgcgcaac ggctacttca tgctgttcaa
      301 cgtatgtctc tttgtctgct tctttgattc tttctaatct cctatctaca taatacacgc
      361 ctggcatttt caaattctta gcgggtttct ttgatcgata agttgtttta agattacata
      421 taagatgccc accaccaact aactttaaag ccatatctgc tctaccctct aatccaccat
      481 cagcaggata tagagtttcc gtacttgctt cccatcccag ggttttcttt tgcataaccg
      541 gtccgttaga agggaagttt acaccacgta ttttcacatt atagattaag cagccgtctt
      601 gtaaacttgt atcttgtgta gcggttaata ctccaccatc ttcatacgta gttacacgtt
      661 cccaagtaaa tccttcagga aaggactgtt taaagaaatc tggaatacct tgagtatgat
      721 ttatgaacgt tttgctccca tacataaacg aagtagctag aatatcaaaa gcaaacggca
      781 atggtccgcc ttcaactg
//
