LOCUS       sequence-164148-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 with sgRNA targeting Exon 1 of VEGF-A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Yiu G, Tieu E, Nguyen AT, Wong B, Smit-McBride Z
  TITLE     Genomic Disruption of VEGF-A Expression in Human Retinal Pigment 
            Epithelial Cells Using CRISPR-Cas9 Endonuclease.
  JOURNAL   Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5490-5497. doi: 
            10.1167/iovs.16-20296.
  PUBMED    27768202
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1167/iovs"; 
            journalName: "Invest Ophthalmol Vis Sci"; date: "2016-10-1- 1"; 
            volume: "57"; issue: "13"; pages: "5490-5497"
COMMENT     Sequence Label: LCV2 V-2
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        208..283
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        345..556
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      575..584
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccggca cttctcgcgg ctccgctgtt ttagagctag aaatagcaag ttaaaataag
      241 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg aattcgctag
      301 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
      361 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag
      421 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      481 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      541 tgccgccaga acacaggacc ggttctagag cgctgccacc atggacaaga agtacagcat
      601 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
      661 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
      721 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
      781 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
      841 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
      901 ggaagaggat aagaa
//
