LOCUS       sequence-164151-        1721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For methylating EcoRI recognition sites in order to prevent cleavage
            by the EcoRI restriction enzyme. AmilCP is a blue reporter for 
            Methylase expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 35)
  AUTHORS   Hendrix J, Read T, Lalonde JF, Jensen PK, Heymann W, Lovelace E, 
            Zimmermann SA, Brasino M, Rokicki J, Dowell RD
  TITLE     Engineered calcium-precipitable restriction enzyme.
  JOURNAL   ACS Synth Biol. 2014 Dec 19;3(12):969-71. doi: 10.1021/sb500042m.
  PUBMED    25524101
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "ACS Synth Biol"; date: "2014-12-19- 19"; volume: "3"; issue: "12"; 
            pages: "969-71"
COMMENT     Sequence Label: pSB1A3 EcoRI Methylase-AmilCP
FEATURES             Location/Qualifiers
     source          1..1721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             44..55
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccggc gcacgcaacg cgaccaataa gctcctgcac aaagcgaaaa aaagcaaaag
      121 cgatgagttt tatacccagt attgcgacat tgaaaacgaa ttacaatact accgcgaaca
      181 tttcagcgat aaggttgtgt actgcaactg tgatgacccg cgtgtgagca atttttttaa
      241 atatttcgcg gtgaactttg acaacctcgg actgaagaag ctgattgcaa gctgttatgt
      301 agaaaacaag gagggcttta gctccagcga agcagcaaaa aatggctttt actatgaata
      361 ccataaagaa aacggcaaaa aactggtctt cgatgatatt tcggtgagta gcttctgtgg
      421 cgacggagat tttcggagca gtgaaagcat tgatctgctc aaaaaaagtg atatcgtggt
      481 cacgaacccg cctttttcac tctttcgtga atacttagat caacttatta aatacgataa
      541 aaagttcctt atcatcgcca atgtaaacag cattacttat aaagaagtgt ttaatctgat
      601 caaagaaaac aagatctggc tgggagtaca tttaggccgg ggtgtgagtg gcttcatcgt
      661 tccagaacat tacgaactgt acggcaccga ggcccgcatt gattcaaatg gaaaccgtat
      721 tatcagcccg aataactgtc tgtggctgac gaaccttgac gtcttcattc gccacaaaga
      781 cctgccgtta acacgtaaat actttggaaa cgaaagctcg tacccaaagt atgataacta
      841 tgatgcgatt aacgttaaca agacgaagga tattccgctg gattataatg gcgttatggg
      901 ggtgccgatc accttcctgc ataaattcaa tcctgaacaa tttgaattga ttaaattccg
      961 gaaaggtgtg gatgagaaag acctatcaat taacggaaag tgcccatatt ttcgaatcct
     1021 gattaaaaat aagcgcttac agaaaaccgg cagtgtgatc gctaaacaaa tgacctacaa
     1081 ggtttatatg tcaggcacgg tcaatggaca ctactttgag gtcgaaggcg atggaaaagg
     1141 taagccctac gagggggagc agacggtaaa gctcactgtc accaagggcg gacctctgcc
     1201 atttgcttgg gatattttat caccacagtg tcagtacgga agcataccat tcaccaagta
     1261 ccctgaagac atccctgact atgtaaagca gtcattcccg gagggctata catgggagag
     1321 gatcatgaac tttgaagatg gtgcagtgtg tactgtcagc aatgattcca gcatccaagg
     1381 caactgtttc atctaccatg tcaagttctc tggtttgaac tttcctccca atggacctgt
     1441 catgcagaag aagacacagg gctgggaacc caacactgag cgtctctttg cacgagatgg
     1501 aatgctgcta ggaaacaact ttatggctct gaagttagaa ggaggcggtc actatttgtg
     1561 tgaatttaaa actacttaca aggcaaagaa gcctgtgaag atgccagggt atcactatgt
     1621 tgaccgcaaa ctggatgtaa ccaatcacaa caaggattac acttcggttg agcagtgtga
     1681 aatttccatt gcacgcaaac ctgtggtcgc caccggttaa t
//
