LOCUS       sequence-164152-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing ALCAM-p2A-E2Crimson.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, 
            Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, 
            Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, 
            Hacohen N, Walker BD
  TITLE     A genome-wide CRISPR screen identifies a restricted set of HIV host 
            dependency factors.
  JOURNAL   Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741.
  PUBMED    27992415
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2016 Dec 19. doi: 10.1038/ng.3741."
COMMENT     Sequence Label: ALCAM-p2A-E2Crimson
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..91)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gcatgaaggg cttgatgacg ttctcagtgc tattggggcc ggggttctcc tccacgtcgc
       61 cggcctgctt cagcaggctg aaatttgtag ctcctccacc tcctgcggcc gcggcctctg
      121 tcttgtggtt gttctcttcc agcttcttat tttcctccat gtttccaagg tccttattga
      181 catgcttgct agcggttttg ctctttttca tatacagcca gtacacaacg cctgcaacca
      241 gagccgccag cagcaacccc accactatgc ccactatgag cttagcttga tcattcacct
      301 tttcgcggtt ctcatcgctg atctcgtcgg cctcgtcatg ctcgggaata ctgatagcgg
      361 aaacgttcag tgaattaact gtgcgctcca actggttctc tgctgtacag gtcagcgtga
      421 cgttttcctc cggtgaaatg atgattttag agtagtatct gccattaatg tacggagatt
      481 cctcggtctg attaatgaca ctgccgctgc ctgtgattgt ccactggatg gccggcttag
      541 ggaacccctc cacgtggcag atgattgttt tagagagacc gctgggatca gtttttttgg
      601 tcatcttgat ctggggtttc ccctccacga tcaatgtcaa actctctctt tttttaaggc
      661 cctcaacctc ctgcagagcg gtctcgcaaa cgtagttccc ggcgtcctgg tagtggagac
      721 tactaaagga aggactgctt ctgagcctga tattgtcctt catccagact actgtcgcat
      781 ttctagaggc agaaattgta catgacacag gaagggcgtc tccgatctgc ctggtgactt
      841 cccctgatgg attcaaagac aggtccagat agtgcacggt gattgcggtg gaagcaatca
      901 tgctcttttt gtcgatcagt gagcacttgt aatcgcctgt agcgtttctc cggacatctg
      961 tcagggtata cgtattgctg cttctgatgc c
//
