LOCUS       sequence-164155-         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the native atoDAEB promoter (Pato) from 
            E. coli DH5A. OG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoG-OG241
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    253..324
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(369..505)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(535..581)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
       61 gccttttgct ggccttttgc tcacatgatt taaatgaaac tcgtaatcgg agaacgggtg
      121 caatagcaag gaccagatcg aacttgactg acgcaattct gagcgtagtc gaaacccaga
      181 caagtcgatc tcgccaagta gctgacttga ctggttaatt aagtttcgat agcccaaggt
      241 aaccaatgag attagggagg gaagaaagtt tgatgaacta cttctgaccc ctagtggtgt
      301 ccagaaaaga ccattaaagg aatgtcttga tcgaaatcga gagatcgcct gcaggatagc
      361 tgacgacctt aaggataaat ttctggtaag gaggacacgt atggaagtgg gcaagttggg
      421 gaagccgtat ccgttgctga atctggcata tgtgggagta taagacgcgc agcgtcgcat
      481 caggcatttt tttctgcgcc aatgccggta gatagcaatc cggcccgagg ggcacaaaaa
      541 acccctcaag acccgtttag aggccccaag gggttatgct acgataccga tagagatggg
      601 ccaataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccc
//
