LOCUS       sequence-164157-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NOT induced by acetoacetate to express GFP. Contains the native 
            atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP
            gene. OG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoK-OG241
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatgtataaa ctccaggaat acggtatcga tccggcggat gtataacacc aaaacttgct
       61 atgcagaaat ttgcacagtg cgcaattttc tgcatagccg ctcattctcc ttataaatcc
      121 ccatccaatt tatcccttca tattcaatta gttaaataac taaatccaat aatctcattc
      181 tggcactccc cttgctattg cctgactgta cccacaacgg tgtatgcaag agggataaaa
      241 aatgaaaaca aaattgatga cattacaaga cgccaggtac ccaccatgac agctctgaca
      301 gagggagcga aactgttcga gaaggagatc ccgtacatca cagagttgga aggagatgtc
      361 gaagggatga aattcatcat caagggagaa ggaacgggtg acgcaacgac cggaacgatc
      421 aaggccaaat acatttgtac cacgggcgat ttgcctgtcc cctgggcgac gcttgtaagc
      481 acgctctcgt atggtgtcca gtgcttcgcg aaatatccat cgcacattaa ggactttttc
      541 aagtcggcca tgccagaagg ttacacacaa gaacgaacca tctcctttga gggggacgga
      601 gtgtataaga cacgcgcgat ggtaacgtac gagcgcgggt ccatctacaa cagggtaact
      661 cttactgggg agaactttaa gaaggacggg catatcttgc ggaaaaacgt ggcatttcaa
      721 tgtccgccct cgattctgta tattctcccg gacacggtga ataatgg
//
