LOCUS       sequence-164158-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NOT induced by acetoacetate to express GFP. Contains the native 
            atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP
            gene. OG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoK-OG241
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    280..351
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(396..532)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(562..608)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
       61 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgatttaa
      121 atgaaactcg taatcggaga acgggtgcaa tagcaaggac cagatcgaac ttgactgacg
      181 caattctgag cgtagtcgaa acccagacaa gtcgatctcg ccaagtagct gacttgactg
      241 gttaattaag tttcgatagc ccaaggtaac caatgagatt agggagggaa gaaagtttga
      301 tgaactactt ctgaccccta gtggtgtcca gaaaagacca ttaaaggaat gtcttgatcg
      361 aaatcgagag atcgcctgca ggatagctga cgaccttaag gataaatttc tggtaaggag
      421 gacacgtatg gaagtgggca agttggggaa gccgtatccg ttgctgaatc tggcatatgt
      481 gggagtataa gacgcgcagc gtcgcatcag gcattttttt ctgcgccaat gccggtagat
      541 agcaatccgg cccgaggggc acaaaaaacc cctcaagacc cgtttagagg ccccaagggg
      601 ttatgctacg ataccgatag agatgggcca ataccacatt tgtagaggtt ttacttgctt
      661 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
      721 ttaacttgtt tattgcagct tataatggtt acaaataa
//
