LOCUS       sequence-164159-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the atoDAEB promoter (Pato) with a 
            strong RBS, Bba_B0034 (iGEM). OG241 backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoL-OG241
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             264..275
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tttctctgtc gaccaactag tgccgtgcat tgatgtataa actccaggaa tacggtatcg
       61 atccggcgga tgtataacac caaaacttgc tatgcagaaa tttgcacagt gcgcaatttt
      121 ctgcatagcc gctcattctc cttataaatc cccatccaat ttatcccttc atattcaatt
      181 agttaaataa ctaaatccaa taatctcatt ctggcactcc ccttgctatt gcctgactgt
      241 acccacaacg gtgtatgcag attaaagagg agaaatacta gatggtaccc accatgacag
      301 ctctgacaga gggagcgaaa ctgttcgaga aggagatccc gtacatcaca gagttggaag
      361 gagatgtcga agggatgaaa ttcatcatca agggagaagg aacgggtgac gcaacgaccg
      421 gaacgatcaa ggccaaatac atttgtacca cgggcgattt gcctgtcccc tgggcgacgc
      481 ttgtaagcac gctctcgtat ggtgtccagt gcttcgcgaa atatccatcg cacattaagg
      541 actttttcaa gtcggccatg ccagaaggtt acacacaaga acgaaccatc tcctttgagg
      601 gggacggagt gtataagaca cgcgcgatgg taacgtacga gcgcgggtcc atctacaaca
      661 gggtaactct tactggggag aactttaaga aggacgggca tatcttgcgg aaaaacgtgg
      721 catttcaatg tccgccctcg at
//
