LOCUS       sequence-164161-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Induced by acetoacetate to express GFP using atoSC two-component 
            system in E. coli. Contains the atoDAEB promoter (Pato) with a 
            strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, 
            Donovan E, Ozdemir T, Thomas GM, Barnes CP
  TITLE     Engineered acetoacetate-inducible whole-cell biosensors based on the
            AtoSC two-component system.
  JOURNAL   Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 
            10.1002/bit.27897. Epub 2021 Aug 9.
  PUBMED    34289076
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.27897";
            journalName: "Biotechnol Bioeng"; date: "2021-11"; volume: "118"; 
            issue: "11"; pages: "4278-4289"
COMMENT     Sequence Label: PatoM-OG241
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttctctgtcg accaactagt gccgtgcatt gatgtataaa ctccaggaat acggtatcga
       61 tccggcggat gtataacacc aaaacttgct atgcagaaat ttgcacagtg cgcaattttc
      121 tgcatagccg ctcattctcc ttataaatcc ccatccaatt tatcccttca tattcaatta
      181 gttaaataac taaatccaat aatctcattc tggcactccc cttgctattg cctgactgta
      241 cccacaacgg tgtatgcaga attcaaagga ggtacccacc atgacagctc tgacagaggg
      301 agcgaaactg ttcgagaagg agatcccgta catcacagag ttggaaggag atgtcgaagg
      361 gatgaaattc atcatcaagg gagaaggaac gggtgacgca acgaccggaa cgatcaaggc
      421 caaatacatt tgtaccacgg gcgatttgcc tgtcccctgg gcgacgcttg taagcacgct
      481 ctcgtatggt gtccagtgct tcgcgaaata tccatcgcac attaaggact ttttcaagtc
      541 ggccatgcca gaaggttaca cacaagaacg aaccatctcc tttgaggggg acggagtgta
      601 taagacacgc gcgatggtaa cgtacgagcg cgggtccatc tacaacaggg taactcttac
      661 tggggagaac tttaagaagg acgggcatat cttgcggaaa aacgtggcat ttcaatgtcc
      721 gccctcgatt ctgtatattc tcccggacac ggtgaataat gggatcagag tggagttcaa
      781 ccaggcatac gatattgagg gtgtgactga a
//
